FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9693_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9693_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567621
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG163391.0422799898700006No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA92540.5903212574978264No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA81640.5207891448251841No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACA80880.5159410342168164No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT77080.49170048117497783No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA73480.46873574671428875No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT67260.4290577888405424No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC57390.3660961418608197No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAA49630.3165943809122231No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA48820.31142731565856796No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG47800.30492064089470605No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA44800.2857833621774651No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT43810.2794680602007756No Hit
GCATTAATGTTACAACCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT43250.27589576817355727No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT42180.269070138764408No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG41640.26562542859530464No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA40990.26147901820656905No Hit
GTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTG40590.25892738104427027No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA40470.25816188989558064No Hit
GAATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTA39980.2550361343717646No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT39490.2519103788479486No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38760.24725364102675326No Hit
GTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATTAAA38100.24304343970896025No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA36850.23506957357677652No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35550.22677675279930545No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC35240.22479923399852386No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT34840.22224759683622508No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT34380.2193132140995815No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC34100.21752706808597233No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33330.21261516654854712No Hit
CACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAA32840.20948941102473112No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT32790.20917045637944376No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG31540.20119659024726003No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT31260.19941044423365087No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA31080.1982622075106164No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG31020.1978794619362716No Hit
CAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGTGA30500.19456233362528316No Hit
CACGTGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC30370.19373305154753603No Hit
GAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAG29020.1851212761247776No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT28920.1844833668342029No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA28430.18135761131038688No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC28310.18059212016169726No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28290.1804645383035823No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT27580.17593538234050193No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT27530.1756164276952146No Hit
GCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTA27440.17504230933369735No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA27130.17306479053291582No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT26470.1688545892151228No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT25930.16540987904601942No Hit
CAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGC25540.1629220328127781No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25390.16196516887691603No Hit
TGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGA25090.16005144100519195No Hit
TAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGC23840.15207757487300821No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23750.15150345651149097No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG23740.1514396655824335No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA23710.15124829279526109No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATTA23660.15092933814997375No Hit
GTAGAAACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTA23180.1478673735552152No Hit
CACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC22930.14627260032877845No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22740.1450605726766865No Hit
GGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTCACAAATAG22720.14493299081857158No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA22660.14455024524422677No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22540.14378475409553712No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22500.14352959037930724No Hit
CTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGT22280.1421261899400429No Hit
GCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATT21740.13868147977093953No Hit
CAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAGCAGCAGTT21370.13632121539581316No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21170.13504539681466374No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG21050.13427990566597411No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT20890.1332592508010546No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA20800.13268513243953736No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20690.1319834322199052No Hit
AACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC20660.13179205943273278No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20640.13166447757461786No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAA20440.13038865899346844No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAAT20420.1302610771353535No Hit
CTTTTGACTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG20150.1285387220508018No Hit
CACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGT19700.1256681302432157No Hit
GTTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAA19310.12318028400997434No Hit
CAGCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCA19300.12311649308091688No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAG19290.12305270215185941No Hit
AACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC19270.12292512029374446No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG19230.12266995657751459No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA19050.12152171985448013No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT18860.1203096922023882No Hit
GTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAA18760.11967178291181349No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC18580.11852354618877906No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA18220.11622707274271014No Hit
GGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAG18080.11533399973590555No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAATGCTAGCTA17990.11475988137438832No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT17940.11444092672910097No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA17850.11386680836758376No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT17630.11246340792831942No Hit
ACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCT17530.11182549863774471No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA17410.11106000748905508No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG17390.11093242563094013No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA17300.11035830726942292No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA17040.1086997431139287No Hit
ATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAA16960.10818941568146892No Hit
AATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGA16870.1076152973199517No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG16810.10723255174560688No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC16780.10704117895843446No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC16680.10640326966785978No Hit
TAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTC16480.10512745108671037No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT16360.10436195993802073No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA16320.10410679622179085No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG16300.10397921436367591No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT16300.10397921436367591No Hit
TCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG16150.10302235042781385No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC16140.1029585594987564No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC16080.10257581392441156No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA16060.10244823206629664No Hit
ATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAAAAGTG15970.1018741137047794No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCC100.0070914486144.9986145
GTCCTGG900.0112.823461
CGTGCAA203.8631866E-4108.773233
AGCACGC5600.0107.498891
CCTGGAG950.0106.864933
GCACGCA5700.0105.592732
CACGCAC5850.0102.885223
TAACCTA301.598048E-596.687324
TAGTAAC24450.083.3409042
CGGTGCA353.434246E-582.874852
ATATGCC651.5625119E-978.0936054
CAACGCA6200.077.1766749
ACAACGC6450.074.1853268
CCAAACG702.8139766E-972.49939
AGTAACA28550.071.372523
CTTATAT754.84215E-967.694081
TAAATTG754.8494257E-967.678965
AGCGGAG1009.094947E-1265.263943
GTAGTAA33350.064.591371
GGGTATA808.06358E-963.4632031
Produced by FastQC (version 0.11.5)