FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9693_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9693_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575466
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG164101.041596581582846No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA93120.5910632155819294No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA81990.5204174510906614No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACA81190.5153395884138408No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT77580.4924257330846873No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA73930.4692579846216929No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT67510.4285081366402068No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC57700.366240845565693No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAA49890.31666821118323085No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA49060.3113999286560294No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG47960.30441786747540095No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA45010.2856932488546246No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT43980.2791555006582179No Hit
GCATTAATGTTACAACCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT43480.275981836485205No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT42250.2681746226195932No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG41830.26550874471426233No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA41190.2614464545728058No Hit
GTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTG40750.25865363010055437No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA40690.2582727903997928No Hit
GAATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTA40130.2547182865260183No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT39620.2514811490695451No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38940.24716496579424754No Hit
GTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATTAAA38280.24297572908587045No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA37030.2350415686533381No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35720.22672656852004425No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC35310.2241241638981736No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT35000.2221564921109056No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT34590.219554087489035No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC34250.21739599585138622No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33460.21238160645802576No Hit
CACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAA33010.2095253087023141No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT32990.2093983621353936No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG31720.20133725513594072No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT31310.19873485051407014No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA31240.1982905375298483No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG31100.1974019115614047No Hit
CAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGTGA30630.19441866723877252No Hit
CACGTGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC30590.1941647741049315No Hit
GAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAG29100.18470725486935294No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT29050.18438988845205165No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA28540.18115275099557845No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC28450.1805814914444361No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28380.18013717846021432No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT27690.17575752190145646No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT27650.1755036287676154No Hit
GCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTA27550.17486889593301283No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA27250.17296469742920506No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT26590.168775460720828No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT26020.16515748356359325No Hit
CAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGC25610.16255507894172264No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25540.16211076595750082No Hit
TGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGA25140.15957183461909047No Hit
TAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGC23940.15195504060385945No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23900.1517011474700184No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA23860.15144725433617737No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG23820.1511933612023363No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATTA23730.150622101651194No Hit
GTAGAAACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTA23260.14763885732856183No Hit
CACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC23030.14617897180897588No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22850.14503645270669122No Hit
GGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTCACAAATAG22840.14497297942323098No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA22740.14433824658862837No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22610.143513093903645No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22590.1433861473367245No Hit
CTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGT22360.14192626181713855No Hit
GCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATT21800.13837175794336407No Hit
CAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAGCAGCAGTT21400.1358328266049537No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21320.13532504033727163No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG21170.13437294108536776No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21020.13342084183346387No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA20890.13259568914848052No Hit
AACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC20760.13177053646349715No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20760.13177053646349715No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20750.1317070631800369No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAA20530.1303106509439112No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAAT20490.13005675781007017No Hit
CTTTTGACTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG20250.12853339900702396No Hit
CACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGT19840.12593099438515334No Hit
CAGCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCA19400.12313816991290195No Hit
GTTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAA19370.12294775006252119No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAG19360.12288427677906093No Hit
AACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC19350.12282080349560065No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG19300.12250343707829937No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA19110.12129744469255446No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT18940.12021839887373005No Hit
GTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAA18830.11952019275566722No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC18650.11837767365338256No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA18290.11609263544881325No Hit
GGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAG18170.11533095604729013No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAATGCTAGCTA18130.1150770629134491No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT17970.11406149037808495No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA17950.11393454381116444No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT17660.11209381859081695No Hit
ACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCT17600.1117129788900554No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG17490.11101477277199255No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA17460.11082435292161176No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA17360.11018962008700918No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA17120.10866626128396296No Hit
ATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAA17030.10809500173282063No Hit
AATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGA16930.10746026889821805No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC16920.10739679561475779No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG16870.10707942919745649No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC16750.10631774979593339No Hit
TAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTC16590.10530217726056924No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA16390.10403271159136408No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT16390.10403271159136408No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG16380.10396923830790382No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT16370.10390576502444357No Hit
TCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG16240.1030806123394602No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC16220.10295366577253968No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC16190.10276324592215891No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA16170.10263629935523838No Hit
ATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAAAAGTG16060.10193809323717554No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGC100.007082716145.058381
GCGGAGG100.007082716145.058381
ACACGCA100.0070867506145.030762
GCGGGCA100.0070867506145.030762
CGGTGCA450.0128.916242
TTGTCGA352.755878E-7103.613141
AGCACGC6600.0101.1012951
CACGCAC6800.099.1754463
GCACGCA6700.098.491042
GGCACCC451.0921212E-896.665695145
ACGTGCA750.087.018462
TAGTAAC26200.084.1399842
GCGGTGC801.8189894E-1281.5953451
CAACGCA5950.076.763939
AGTAACA29200.075.7438053
ACAACGC6150.074.267558
CGTGCTA809.822543E-1172.515383
CAGCGTG1000.072.4992757
AACACGT5250.071.822486
TAACAAG31250.071.236855
Produced by FastQC (version 0.11.5)