FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9609_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9609_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103495
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACC74510.6752182837257985No Hit
AAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGG47140.427188161251297No Hit
GGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG46390.42039157404428656No Hit
GCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAG34700.3144554347776836No Hit
GTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAA32660.2959687175746152No Hit
CATATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACA32430.29388443083113197No Hit
AGCGAAAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGG30950.28047249874263136No Hit
AGCGAAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGG28710.26017335828436017No Hit
CATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACATAGTCAGATCTT26140.2368837194550043No Hit
GTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTT24760.2243779989941051No Hit
GCATTATGCCATGGAGGTTCAGATTACGCTCGTCCACTCCTGCTTGCACT24560.2225655757389023No Hit
GTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGAGG24170.21903135039125685No Hit
GGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACA24130.21866886574021632No Hit
AAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGG23310.21123793039388486No Hit
GGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCA22610.20489444900067513No Hit
CATTTAAGGAGCGCAGAGCTGAGAGTCAGCCGGGCGTCTGATTGGTGGCG22170.200907117839229No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC21510.1949261210970598No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT20940.18976071481973186No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGG20150.18260164296168085No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA19540.17707375203331235No Hit
GCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCA19310.17498946528982914No Hit
AGGCTGGAGTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCT19190.17390201133670746No Hit
GGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAAC19180.1738113901739473No Hit
CATATAATGCATACATATGCATTGTAATCATTTTGTGTTAAAAAAAAGAC19060.17272393622082563No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG18800.17036778598906202No Hit
GATTTATATTTTATTGATCATTTCACCTGTGAATAAATTATGCACCGTTG18580.16837412040833896No Hit
CATAAAACAGCGTGCCGGGTCCCAAACAGACGGAACATTCATTTAGGTTT18570.1682834992455788No Hit
GGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG18440.167105424129697No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA18170.16465865273517324No Hit
ATTATGCACCGTTGAAAAAAGGCCCAGGTTTTGCCCAGCTGCCTCACTGC17980.16293685064273059No Hit
ATGCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGAC16300.1477124952990272No Hit
GTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGA15990.14490323925346285No Hit
GTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGT15950.1445407546024223No Hit
GCATAATGCACCCCTGCAGAAGCCCCCTGCATTTGCGTAAGCAGCCACAT15860.14372516413758107No Hit
GTATGTATGTGTGTACCTGAGCTTGCTCTTCAAGGTGTTCACTTCGCGGT15390.1394659694878545No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA15220.13792540972093212No Hit
ATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTCTC15080.1366567134422902No Hit
TATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACACG15070.13656609227953004No Hit
GTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGA15060.13647547111676991No Hit
GTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGT14710.13330373042016502No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTG14660.13285062460636432No Hit
GTTTTAACAACGCTCACAACACAGGGAGATCAGATCAGATCAGAACGTGC14270.12931639925871888No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA13380.12125111577306648No Hit
GTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTGACAGGATCC13320.12070738879650564No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG13230.11989179833166438No Hit
TCACAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCC13000.1178075115881812No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA12980.1176262692626609No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT12870.11662943647229938No Hit
CTGTAGGCAAGCATAAGTGACTTGAACTTAATGCGAGCAGCTACCGGCAG12850.11644819414677911No Hit
GTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTC12690.11499825554261688No Hit
GTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTA12690.11499825554261688No Hit
CCAAATCAACTGTCCGAGCTGCCACCATGTCAGCCGATCACAATACAGGG12660.11472639205433646No Hit
GGATGGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATG12620.1143639074032959No Hit
GCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACAT12440.11273272647361339No Hit
GAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGTGTGTGTGT12380.11218899949705255No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA12360.11200775717153226No Hit
GTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGTTA12290.1113734090322113No Hit
GGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCA12240.11092030321841059No Hit
GTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAAATCAACTGTC12090.1095609857770085No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC11960.10838291066112671No Hit
CAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTC11880.10765794135904558No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA11880.10765794135904558No Hit
GTGTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGG11820.10711421438248474No Hit
CTGTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGA11620.10530179112728197No Hit
CCTCTGACTGTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTG11530.10448620066244071No Hit
GTCTGTGATAATACATCACAGGCGGCACATTGTATATCAGAAATCAATGA11390.10321750438379874No Hit
CTCTTGTGGTGTTTAGGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC11320.10258315624447777No Hit
GGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATGTCAG11260.10203942926791693No Hit
CACATACACACACTCAAACACACACAACCATTTACACACCCTCTCTCCTT11160.10113321764031555No Hit
CATTATAAGAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGT11110.10068011182651485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA17900.0128.051131
GAGCAAA2950.0108.188241
AAGTGCG451.09012035E-896.687425
GCGAAAG24100.095.387872
ATAAGTA1550.088.894083
TATAAGT1550.088.894082
GGGATAC259.368875E-487.042351
CGAAAGC27200.084.516463
TACTGGG1350.080.5545967
GGATAAC406.647445E-572.535291
CGTCAAA300.001932061372.535291
TAATAGT502.3028388E-672.518864
CTAGACG608.0179234E-872.518854
GATCCGC300.001934147172.515575
GATCACC406.663885E-572.49914145
CGGAGCC1550.070.1795354
GCCGACC1550.070.160458
TAGAAAC1600.067.986432
GAGCCGA1600.067.980266
AGCCGAC1600.067.967947
Produced by FastQC (version 0.11.5)