FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9609_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9609_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111201
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACC75090.6757553313936903No Hit
AAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGG47560.4280053743652139No Hit
GGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG46580.4191860878454933No Hit
GCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAG34900.3140745913655585No Hit
GTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAA32880.29589606200858354No Hit
CATATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACA32640.29373623673844784No Hit
AGCGAAAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGG31200.280777285117634No Hit
AGCGAAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGG28910.26016895233175635No Hit
CATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACATAGTCAGATCTT26380.23740079427574307No Hit
GTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTT24850.22363190817862835No Hit
GCATTATGCCATGGAGGTTCAGATTACGCTCGTCCACTCCTGCTTGCACT24720.22246200282397152No Hit
GTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGAGG24360.2192222649187681No Hit
GGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACA24260.21832233772287823No Hit
AAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGG23510.2115728837537043No Hit
GGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCA22790.2050934079432974No Hit
CATTTAAGGAGCGCAGAGCTGAGAGTCAGCCGGGCGTCTGATTGGTGGCG22270.20041378652467015No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC21590.19429428159261916No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT21080.1897046528935809No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGG20240.18214526444810614No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA19660.176925686711945No Hit
GCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCA19410.17467586872222038No Hit
GGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAAC19330.1739559269655085No Hit
AGGCTGGAGTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCT19310.17377594152633052No Hit
CATATAATGCATACATATGCATTGTAATCATTTTGTGTTAAAAAAAAGAC19240.17314599248920762No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG18960.17062619634071605No Hit
CATAAAACAGCGTGCCGGGTCCCAAACAGACGGAACATTCATTTAGGTTT18700.1682863856314024No Hit
GATTTATATTTTATTGATCATTTCACCTGTGAATAAATTATGCACCGTTG18670.16801640747263547No Hit
GGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG18580.1672064729963346No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA18340.16504664772619895No Hit
ATTATGCACCGTTGAAAAAAGGCCCAGGTTTTGCCCAGCTGCCTCACTGC18100.1628868224560633No Hit
ATGCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGAC16400.1475880601259358No Hit
GTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGA16120.1450682639774442No Hit
GTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGT16080.14470829309908828No Hit
GCATAATGCACCCCTGCAGAAGCCCCCTGCATTTGCGTAAGCAGCCACAT15960.14362838046402046No Hit
GTATGTATGTGTGTACCTGAGCTTGCTCTTCAAGGTGTTCACTTCGCGGT15500.13948871536292715No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA15360.13822881728868136No Hit
GTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGA15210.13687892649484656No Hit
ATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTCTC15170.1365189556164906No Hit
TATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACACG15140.13624897745772366No Hit
GTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGT14780.1330092395525202No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTG14730.13255927595457526No Hit
GTTTTAACAACGCTCACAACACAGGGAGATCAGATCAGATCAGAACGTGC14370.12931953804937182No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA13500.12149017144513008No Hit
GTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTGACAGGATCC13410.12068023696882922No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG13290.11960032433376139No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA13130.11816044082033764No Hit
TCACAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCC13130.11816044082033764No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT12990.11690054274609184No Hit
CTGTAGGCAAGCATAAGTGACTTGAACTTAATGCGAGCAGCTACCGGCAG12920.11627059370896894No Hit
GTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTA12810.11528067379349011No Hit
CCAAATCAACTGTCCGAGCTGCCACCATGTCAGCCGATCACAATACAGGG12760.11483071019554518No Hit
GTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTC12750.11474071747595618No Hit
GGATGGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATG12670.11402077571924432No Hit
GCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACAT12540.11285087036458752No Hit
GAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGTGTGTGTGT12510.11258089220582054No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA12460.11213092860787562No Hit
GGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCA12320.11087103053362983No Hit
GTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGTTA12320.11087103053362983No Hit
GTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAAATCAACTGTC12150.10934115430061707No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC11980.10781127806760434No Hit
CAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTC11970.10772128534801534No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA11930.10736131446965941No Hit
GTGTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGG11850.10664137271294753No Hit
CTGTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGA11670.10502150376034579No Hit
CCTCTGACTGTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTG11590.10430156200363391No Hit
GTCTGTGATAATACATCACAGGCGGCACATTGTATATCAGAAATCAATGA11470.10322164936856608No Hit
CTCTTGTGGTGTTTAGGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC11420.10277168577062115No Hit
GGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATGTCAG11290.10160178041596435No Hit
CACATACACACACTCAAACACACACAACCATTTACACACCCTCTCTCCTT11260.10133180225719739No Hit
CATTATAAGAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGT11220.10097183137884144No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGA100.0070827473145.056272
AGCGAAA18800.0123.871381
GAGCAAA2450.0103.639611
GCGAAAG25050.093.229782
GATCACC608.2945917E-1084.58189145
CGAAAGC28550.081.546523
TAGAAAC1300.072.528133
GTAGACT754.840331E-967.6929244
AGTAGAC754.840331E-967.6929243
ATAAGTA1350.064.469463
GGATAAC350.003557321662.1837651
GACATAA1750.062.1837651
TATAAGT1400.062.166972
CAGGTAT350.003566849562.1417929
CAGGCCG3050.059.4252179
GCCAGAC2100.058.713253
TACTGGG1500.057.9990127
AGGCCGT1051.1077645E-955.2520686
GTATAGA400.00603532954.4107931
AAGTGCG553.2144258E-452.7429855
Produced by FastQC (version 0.11.5)