FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9608_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9608_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253849
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCA10570.4163892707869639No Hit
ATGTAATTCTGTGGTAATTTCATGGGCAACATAATCCAATTGTGACCGTT10420.4104802461305737No Hit
GTGTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACA10220.40260154658872005No Hit
GCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTG9790.3856623425737348No Hit
GTAAATAACCAGGCCCACCAATTTGCCTTTCTCTCGCCTCCAAATGATGA8410.3312993157349448No Hit
CTATAGAAGTGTGTGTTGTTGTTGGTGTCTGGGACACAAATATTCGTCAC7890.3108146969261254No Hit
TTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCTGGAA7670.3021481274300864No Hit
AGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAA6660.2623606947437256No Hit
CTTCTATAGCGTTTAAGCGTTTGGACGTAGAACATTTGGAAAACGGTACA6330.2493608404996671No Hit
GGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGT6240.245815425705833No Hit
GTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATC6130.2414821409578135No Hit
CATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGATAG6110.24069427100362814No Hit
CTATTTAAGTTATTTAATTTCTCCTGGAGAGGGAGGAGTGACTGGGAACT5790.22808835173666236No Hit
AGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGA5750.2265126118282916No Hit
AGCGAAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGG5610.2209975221489941No Hit
GGAATGAAAAGCCTCCATCTCGCTTCTGTCCTTTAACAAATGAGCATAAT5610.2209975221489941No Hit
GAATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGA5490.2162703024238819No Hit
AAGCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTG5480.21587636744678923No Hit
TTTCATTCCTGTGCAGCCTTTTCCTGTTTAGTGCTCACATAGTTTGCGAT5460.2150884974926039No Hit
AAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGT5320.20957340781330636No Hit
GTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGGAA5300.20878553785912096No Hit
CTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGG5240.20642192799656486No Hit
CACATGCACCTGCCTTTGTTTCTGACTGTCGGCGATTCTGACAAGGGCAT5180.2040583181340088No Hit
CTATTAATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAG5120.20169470827145272No Hit
ACTTATTCCTGGTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGG5110.20130077329436002No Hit
TATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTATATGA5080.200118968363082No Hit
GTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATC4980.19617961859215516No Hit
ATCTAATGCTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGG4950.19499781366087715No Hit
CATATAGCTTGAGCTGTTTTCACCATGGCCCCGTGCTTGCTAATGTCATG4920.1938160087295991No Hit
ATTTAAGTCTGGACTAAGGTTGAACTTTAACAGAAATGGCAGTCAGGCCA4800.18908878900448692No Hit
GTTTTAAATTGAGGTAAAAATCGCAAACTATGTGAGCACTAAACAGGAAA4780.18830091905030155No Hit
GTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGGGAGAGGCCGA4710.1855433742106528No Hit
GTGTGAGGATTACAAAAACAAAATAAAAAGGGTTGCTTCATCATTTGGAG4690.18475550425646742No Hit
TATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATC4670.18396763430228205No Hit
CTTCCAGGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGG4630.18239189439391135No Hit
CTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGT4630.18239189439391135No Hit
GTCTTACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCT4540.1788464796000772No Hit
CTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGC4530.17845254462298454No Hit
GTATGAGTCTGTCTTTTTAGAAACTGTTTCTTCTAGTGAAATTATGATAT4480.17648286973752111No Hit
GGTTATTTACAGTGACTTTTGCAGACTTTTGCTAAATGTGGAAGATTATG4440.1749071298291504No Hit
CCATTTATGCTGATGTGATTTCACCGCCTGTAAGGTCATGTAGTAGGTGC4420.17411925987496504No Hit
CCTTTGGCTTGGTAATTTAGCACCATAATTGGACGAGGCTGCAGCTGCTT4410.17372532489787235No Hit
TCTCTGCATTGTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGG4330.1705738450811309No Hit
CCATAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCC4310.16978597512694554No Hit
TACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCTAACT4300.16939204014985287No Hit
GGCAATGGCGTGTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGT4260.16781630024148214No Hit
CCCCTACTATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAA4250.16742236526438944No Hit
GTGTGTGTGTGTGTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGC4160.16387695047055534No Hit
GACATACACAGGTATTTCTGCCAAGGGATCAAATCCTTGCGGGACCCACC4090.16111940563090654No Hit
ATCCAAAGGAGCAGGGGCTGCTTTACTCGTGTCCCCGGCACAGTTAATTG4070.1603315356767212No Hit
ACATAATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCAC4020.15836186079125777No Hit
GTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGG4010.1579679258141651No Hit
GTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAATTGTAGGAGTCT3980.15678612088288707No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA3940.15521038097451634No Hit
ATTTAAAACATACGCCATCAATCTTCAAATACACTAAACTTATCCTTTCC3890.15324070608905294No Hit
TCCTCACACAAAGCACCCAAGGCGACCAAGTCCTCCGGATTAGGAAAGTC3860.1520589011577749No Hit
GTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGGAAATTTGAAAAAACA3810.1500892262723115No Hit
CTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAAGCAGCAGCGAGCGC3800.14969529129521883No Hit
GTGAATAGCAGTCCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCAC3790.14930135631812613No Hit
GCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTGCT3770.14851348636394077No Hit
TAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCCGTC3770.14851348636394077No Hit
CTCCTGGAGAGGGAGGAGTGACTGGGAACTCAGACCAGGTGGATTCCTTG3760.14811955138684807No Hit
CTTAAATAGTGTTTCATTGAAATGGATCTCATTCGAAATCATATCCTAAG3710.1461498765013847No Hit
GTATTTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCT3710.1461498765013847No Hit
CTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGGGAGTCGG3690.1453620065471993No Hit
CTGCTGGACTCCTTGATTTTTTTTTAATCATTGTTTGATTTTGAGCAGTA3680.14496807157010663No Hit
ATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATCAT3650.1437862666388286No Hit
GTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGA3640.14339233166173593No Hit
GAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGGAGGAC3600.14181659175336517No Hit
ATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGG3600.14181659175336517No Hit
CGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATTAATAATAATA3590.1414226567762725No Hit
CTATGTGAGCACTAAACAGGAAAAGGCTGCACAGGAATGAAAAGCCTCCA3580.14102872179917983No Hit
GGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTAAGTTAT3500.13787724198243836No Hit
GTACCTGAGTGGTTTCTTCTCAGTCTTCACGCTCTCAACTGTGTCAAGCT3490.1374833070053457No Hit
CTATTGTTTTTGTACCGTTTTCCAAATGTTCTACGTCCAAACGCTTAAAC3390.1335439572344189No Hit
GTGAAACACTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCA3380.13315002225732622No Hit
ATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCACACGTA3360.13236215230314083No Hit
GGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGGAAAAGA3350.13196821732604816No Hit
CAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTT3340.13157428234895546No Hit
GTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTAT3340.13157428234895546No Hit
ACCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCA3320.13078641239477012No Hit
CCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCAT3310.13039247741767745No Hit
ATTCTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGG3290.1296046074634921No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT3290.1296046074634921No Hit
ATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAA3260.12842280253221403No Hit
GCCTTTGAACGTGTGTGTGTGTGTGTGTGTGCGTACGTGTGCTAGTTTGT3260.12842280253221403No Hit
GAGTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTAT3220.12684706262384332No Hit
CTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGC3150.12408951778419455No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC3060.12054410299036042No Hit
GTCATGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAA3060.12054410299036042No Hit
GTGCTGGGAGGTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGAC3030.11936229805908237No Hit
TCAATTAACTGTGCCGGGGACACGAGTAAAGCAGCCCCTGCTCCTTTGGA3010.118574428104897No Hit
GTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGG2980.11739262317361895No Hit
TTCTTAAACTCTCTAATTTTCCCTGTCCACATTACAACGCAAACCCGGCG2960.1166047532194336No Hit
ATGTTACCCTGGTTACTTTTTAGAACCCACTGAAATGCCTCTGCATGCAT2950.11621081824234093No Hit
GTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGGTAAAAA2950.11621081824234093No Hit
ATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAATTTCC2950.11621081824234093No Hit
GTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCAC2930.11542294828815555No Hit
GTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGTGT2910.11463507833397019No Hit
TTCCTAATCCGGAGGACTTGGTCGCCTTGGGTGCTTTGTGTGAGGATTAC2900.11424114335687752No Hit
ATCAAAGACAACTTCTTCTCAATATTATTTTTTTGAGCCCACATGCACCT2900.11424114335687752No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG2900.11424114335687752No Hit
CATCATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAAT2890.11384720837978483No Hit
GCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGAACCACAAC2870.11305933842559947No Hit
ATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAATCAGACGA2870.11305933842559947No Hit
CCTATGAACCACAACTCAACTTAATTCATCATTGGCTTAGGATATGATTT2860.1126654034485068No Hit
GTCCTGGAAACACATCAAGCTCAGCTCCTGTGTCCAGCTCGCTTCTCTGC2860.1126654034485068No Hit
CTCCAGATAGGACCAGGAATAAGTTGTCTGGGCTTTTATTTCCCCCCACC2850.1122714684714141No Hit
GGCTTAGGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTA2840.11187753349432142No Hit
CCTTTGGATAAGGCAGTGAGGGAGAACAGTGCTGGGAGGTTGTATAGAAT2830.11148359851722875No Hit
ATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAAT2830.11148359851722875No Hit
TGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAAAGGG2830.11148359851722875No Hit
ATCATATCCTAAGCCAATGATGAATTAAGTTGAGTTGTGGTTCATAGGAG2820.11108966354013608No Hit
GTGTGTGTGTGTGCGTACGTGTGCTAGTTTGTGTGTGGTGGTTTTTTTAA2820.11108966354013608No Hit
CCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGG2810.11069572856304338No Hit
GTTCTAGCGCCTTCGACTTTACCATTATCCCGCCTGTTAAGGAAAGAAAA2780.10951392363176533No Hit
GTACAGGAACAGTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAAT2770.10911998865467266No Hit
ATGCAGAGAGAAGCAGCTGCAGCCTCGTCCAATTATGGTGCTAAATTACC2770.10911998865467266No Hit
GAAATAAACAGGGAGAGAGAGGGAATATTACAGGTGGTCCTATCTGCCTA2770.10911998865467266No Hit
GCAAGAAACAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCA2750.10833211870048728No Hit
CTCTATGGGCTGACATTTTACCTCATTATCTGAAATTGGATACTGGAACC2740.10793818372339462No Hit
AGCGAAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGC2740.10793818372339462No Hit
TACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGG2730.10754424874630195No Hit
ACCATGGACAATAATGAGACTATTTCTTTTCTTTTCTATTCTTTTCTTGT2720.10715031376920925No Hit
GCTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAAT2720.10715031376920925No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT2710.10675637879211657No Hit
GTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGCACCATAATTGGACG2690.10596850883793123No Hit
GTCCACACCTATTCATCCATGGTGAGTTTGGATTCACGTGTTACCAGAAT2680.10557457386083853No Hit
GGGTAAAATCATGCCCTTGTCAGAATCGCCGACAGTCAGAAACAAAGGCA2680.10557457386083853No Hit
GTATAGCGAGTGGCGACAAGCTCATAATGGCATATAACTGTAGCAAATAC2670.10518063888374585No Hit
AAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGA2670.10518063888374585No Hit
CGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACG2630.10360489897537513No Hit
GTTATATGCCATTATGAGCTTGTCGCCACTCGCTATACAGCCCAACAAAC2630.10360489897537513No Hit
CTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATT2630.10360489897537513No Hit
TCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGCAGGGGCTGCT2620.10321096399828243No Hit
CAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGGTCAATGTA2620.10321096399828243No Hit
TAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTGTGTGTGT2610.10281702902118976No Hit
GGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAA2600.10242309404409708No Hit
TGCCTACACCGCCTCATAATAATTGATATTCATGACTATTAATATTACAC2570.10124128911281904No Hit
AAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATT2560.10084735413572636No Hit
CTCATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGAT2550.10045341915863368No Hit
CCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAATTTCCAAACAGCAAT2550.10045341915863368No Hit
CTCTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTA2540.100059484181541No Hit
ACTATAGCCTCTCCAGCAGAGCTTTCTATGGGCTGAGAGCCACTGAATGC2540.100059484181541No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA301.09679604E-7120.8798141
AGCGAAA1900.0118.334981
CAAGATT451.0884833E-896.665747
GCGAAAG2450.091.75192
ACAAGAT502.258821E-887.016316
CCATGCC353.4324887E-582.856359
CGAAAGC2800.080.282913
GCAGGAC554.375397E-879.105743
AGGCAAT651.5552359E-978.091576
GAAAGCA3000.074.930714
GAGTGGT1650.074.696267
GGCAATG1400.072.5278851
GGTGTAA300.001931123772.5278851
ACCTGAG1700.072.5135963
TACCTGA1700.072.5135962
CAGGACG607.997187E-872.5135964
ATGACTT406.6539316E-572.499319
AGTGGTT1700.072.499318
GATGACT406.6539316E-572.499318
CCCATGC300.001934145972.4993068
Produced by FastQC (version 0.11.5)