FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9608_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9608_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255531
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCA10630.4159964935761219No Hit
ATGTAATTCTGTGGTAATTTCATGGGCAACATAATCCAATTGTGACCGTT10460.4093436804145094No Hit
GTGTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACA10270.4019081833515307No Hit
GCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTG9850.3854718214228411No Hit
GTAAATAACCAGGCCCACCAATTTGCCTTTCTCTCGCCTCCAAATGATGA8450.3306839483272088No Hit
CTATAGAAGTGTGTGTTGTTGTTGGTGTCTGGGACACAAATATTCGTCAC7920.3099428249410052No Hit
TTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCTGGAA7710.3017246439766604No Hit
AGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAA6710.26259044890835165No Hit
CTTCTATAGCGTTTAAGCGTTTGGACGTAGAACATTTGGAAAACGGTACA6340.24811079673307737No Hit
GGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGT6280.24576274502897885No Hit
GTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATC6170.2414579835714649No Hit
CATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGATAG6130.23989261576873255No Hit
CTATTTAAGTTATTTAATTTCTCCTGGAGAGGGAGGAGTGACTGGGAACT5800.2269783313961907No Hit
AGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGA5780.22619564749482451No Hit
AGCGAAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGG5670.22189088603731055No Hit
GGAATGAAAAGCCTCCATCTCGCTTCTGTCCTTTAACAAATGAGCATAAT5650.22110820213594437No Hit
GAATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGA5540.21680344067843044No Hit
AAGCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTG5500.21523807287569807No Hit
TTTCATTCCTGTGCAGCCTTTTCCTGTTTAGTGCTCACATAGTTTGCGAT5480.2144553889743319No Hit
AAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGT5350.20936794361545177No Hit
GTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGGAA5320.2081939177634025No Hit
CTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGG5250.2054545241086209No Hit
CACATGCACCTGCCTTTGTTTCTGACTGTCGGCGATTCTGACAAGGGCAT5200.20349781435520542No Hit
CTATTAATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAG5160.2019324465524731No Hit
ACTTATTCCTGGTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGG5110.19997573679905764No Hit
TATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTATATGA5100.19958439484837456No Hit
GTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATC5030.19684500119359294No Hit
ATCTAATGCTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGG5000.19567097534154368No Hit
CATATAGCTTGAGCTGTTTTCACCATGGCCCCGTGCTTGCTAATGTCATG4940.19332292363744516No Hit
ATTTAAGTCTGGACTAAGGTTGAACTTTAACAGAAATGGCAGTCAGGCCA4830.1890181621799312No Hit
GTTTTAAATTGAGGTAAAAATCGCAAACTATGTGAGCACTAAACAGGAAA4820.18862682022924812No Hit
GTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGGGAGAGGCCGA4750.1858874265744665No Hit
GTGTGAGGATTACAAAAACAAAATAAAAAGGGTTGCTTCATCATTTGGAG4700.18393071682105108No Hit
TATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATC4690.18353937487036798No Hit
CTTCCAGGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGG4670.1827566909690018No Hit
CTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGT4630.18119132316626946No Hit
GTCTTACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCT4590.1796259553635371No Hit
CTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGC4530.17727790365943857No Hit
GTATGAGTCTGTCTTTTTAGAAACTGTTTCTTCTAGTGAAATTATGATAT4510.17649521975807242No Hit
CCTTTGGCTTGGTAATTTAGCACCATAATTGGACGAGGCTGCAGCTGCTT4470.17492985195534005No Hit
CCATTTATGCTGATGTGATTTCACCGCCTGTAAGGTCATGTAGTAGGTGC4450.17414716805397387No Hit
GGTTATTTACAGTGACTTTTGCAGACTTTTGCTAAATGTGGAAGATTATG4440.1737558261032908No Hit
TCTCTGCATTGTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGG4360.1706250904978261No Hit
CCATAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCC4310.16866838074441065No Hit
TACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCTAACT4310.16866838074441065No Hit
GGCAATGGCGTGTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGT4290.1678856968430445No Hit
CCCCTACTATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAA4270.1671030129416783No Hit
GTGTGTGTGTGTGTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGC4190.1639722773362136No Hit
GACATACACAGGTATTTCTGCCAAGGGATCAAATCCTTGCGGGACCCACC4110.1608415417307489No Hit
ATCCAAAGGAGCAGGGGCTGCTTTACTCGTGTCCCCGGCACAGTTAATTG4080.15966751587869965No Hit
ACATAATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCAC4040.15810214807596729No Hit
GTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGG4040.15810214807596729No Hit
GTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAATTGTAGGAGTCT3990.15614543832255187No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA3970.1553627544211857No Hit
ATTTAAAACATACGCCATCAATCTTCAAATACACTAAACTTATCCTTTCC3910.15301470271708717No Hit
TCCTCACACAAAGCACCCAAGGCGACCAAGTCCTCCGGATTAGGAAAGTC3870.1514493349143548No Hit
GTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGGAAATTTGAAAAAACA3840.15027530906230557No Hit
CTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAAGCAGCAGCGAGCGC3830.14988396711162247No Hit
GTGAATAGCAGTCCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCAC3810.14910128321025629No Hit
TAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCCGTC3800.1487099412595732No Hit
GCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTGCT3790.14831859930889013No Hit
CTCCTGGAGAGGGAGGAGTGACTGGGAACTCAGACCAGGTGGATTCCTTG3770.14753591540752395No Hit
CTTAAATAGTGTTTCATTGAAATGGATCTCATTCGAAATCATATCCTAAG3730.14597054760479158No Hit
GTATTTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCT3730.14597054760479158No Hit
CTGCTGGACTCCTTGATTTTTTTTTAATCATTGTTTGATTTTGAGCAGTA3710.1451878637034254No Hit
CTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGGGAGTCGG3700.14479652175274232No Hit
ATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATCAT3660.14323115395001No Hit
GTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGA3650.1428398119993269No Hit
GAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGGAGGAC3620.14166578614727762No Hit
ATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGG3620.14166578614727762No Hit
CGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATTAATAATAATA3620.14166578614727762No Hit
CTATGTGAGCACTAAACAGGAAAAGGCTGCACAGGAATGAAAAGCCTCCA3590.14049176029522836No Hit
GGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTAAGTTAT3530.13814370859112984No Hit
GTACCTGAGTGGTTTCTTCTCAGTCTTCACGCTCTCAACTGTGTCAAGCT3490.13657834078839748No Hit
CTATTGTTTTTGTACCGTTTTCCAAATGTTCTACGTCCAAACGCTTAAAC3420.13383894713361588No Hit
GTGAAACACTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCA3400.1330562632322497No Hit
CAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTT3380.13227357933088352No Hit
CCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCAT3370.13188223738020044No Hit
ATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCACACGTA3370.13188223738020044No Hit
GGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGGAAAAGA3370.13188223738020044No Hit
ACCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCA3350.13109955347883426No Hit
GTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTAT3340.13070821152815118No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT3310.12953418567610192No Hit
ATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAA3300.12914284372541884No Hit
ATTCTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGG3300.12914284372541884No Hit
GCCTTTGAACGTGTGTGTGTGTGTGTGTGTGCGTACGTGTGCTAGTTTGT3280.12836015982405266No Hit
GAGTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTAT3220.12601210811995414No Hit
CTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGC3150.12327271446517252No Hit
GTCATGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAA3090.120924662761074No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC3060.11975063690902474No Hit
TCAATTAACTGTGCCGGGGACACGAGTAAAGCAGCCCCTGCTCCTTTGGA3030.11857661105697548No Hit
GTGCTGGGAGGTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGAC3030.11857661105697548No Hit
GTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGGTAAAAA2990.11701124325424311No Hit
GTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGG2990.11701124325424311No Hit
TTCTTAAACTCTCTAATTTTCCCTGTCCACATTACAACGCAAACCCGGCG2980.11661990130356004No Hit
ATGTTACCCTGGTTACTTTTTAGAACCCACTGAAATGCCTCTGCATGCAT2970.11622855935287695No Hit
ATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAATTTCC2960.11583721740219385No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG2930.11466319155014461No Hit
GTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCAC2930.11466319155014461No Hit
ATCAAAGACAACTTCTTCTCAATATTATTTTTTTGAGCCCACATGCACCT2920.11427184959946152No Hit
CCTATGAACCACAACTCAACTTAATTCATCATTGGCTTAGGATATGATTT2910.11388050764877843No Hit
GTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGTGT2910.11388050764877843No Hit
ATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAATCAGACGA2910.11388050764877843No Hit
CATCATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAAT2910.11388050764877843No Hit
TTCCTAATCCGGAGGACTTGGTCGCCTTGGGTGCTTTGTGTGAGGATTAC2910.11388050764877843No Hit
GCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGAACCACAAC2890.11309782374741226No Hit
GTCCTGGAAACACATCAAGCTCAGCTCCTGTGTCCAGCTCGCTTCTCTGC2880.11270648179672917No Hit
CCTTTGGATAAGGCAGTGAGGGAGAACAGTGCTGGGAGGTTGTATAGAAT2870.11231513984604607No Hit
CTCCAGATAGGACCAGGAATAAGTTGTCTGGGCTTTTATTTCCCCCCACC2860.11192379789536298No Hit
GGCTTAGGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTA2860.11192379789536298No Hit
ATCATATCCTAAGCCAATGATGAATTAAGTTGAGTTGTGGTTCATAGGAG2840.11114111399399682No Hit
ATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAAT2830.11074977204331372No Hit
GTGTGTGTGTGTGCGTACGTGTGCTAGTTTGTGTGTGGTGGTTTTTTTAA2830.11074977204331372No Hit
TGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAAAGGG2830.11074977204331372No Hit
CCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGG2810.10996708814194754No Hit
GAAATAAACAGGGAGAGAGAGGGAATATTACAGGTGGTCCTATCTGCCTA2800.10957574619126445No Hit
GTACAGGAACAGTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAAT2790.10918440424058137No Hit
GTTCTAGCGCCTTCGACTTTACCATTATCCCGCCTGTTAAGGAAAGAAAA2790.10918440424058137No Hit
ATGCAGAGAGAAGCAGCTGCAGCCTCGTCCAATTATGGTGCTAAATTACC2780.10879306228989828No Hit
GCAAGAAACAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCA2770.10840172033921522No Hit
CTCTATGGGCTGACATTTTACCTCATTATCTGAAATTGGATACTGGAACC2760.10801037838853213No Hit
AGCGAAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGC2750.10761903643784904No Hit
GCTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAAT2740.10722769448716594No Hit
ACCATGGACAATAATGAGACTATTTCTTTTCTTTTCTATTCTTTTCTTGT2730.10683635253648285No Hit
TACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGG2730.10683635253648285No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT2710.10605366863511669No Hit
GTCCACACCTATTCATCCATGGTGAGTTTGGATTCACGTGTTACCAGAAT2710.10605366863511669No Hit
GTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGCACCATAATTGGACG2710.10605366863511669No Hit
GTATAGCGAGTGGCGACAAGCTCATAATGGCATATAACTGTAGCAAATAC2700.1056623266844336No Hit
GGGTAAAATCATGCCCTTGTCAGAATCGCCGACAGTCAGAAACAAAGGCA2700.1056623266844336No Hit
AAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGA2690.1052709847337505No Hit
CTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATT2660.10409695888170124No Hit
CAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGGTCAATGTA2650.10370561693101815No Hit
GTTATATGCCATTATGAGCTTGTCGCCACTCGCTATACAGCCCAACAAAC2650.10370561693101815No Hit
TCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGCAGGGGCTGCT2630.10292293302965197No Hit
TGCCTACACCGCCTCATAATAATTGATATTCATGACTATTAATATTACAC2630.10292293302965197No Hit
CGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACG2630.10292293302965197No Hit
GGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAA2630.10292293302965197No Hit
TAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTGTGTGTGT2620.10253159107896888No Hit
AAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATT2590.10135756522691965No Hit
CTCATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGAT2570.10057488132555346No Hit
ACTATAGCCTCTCCAGCAGAGCTTTCTATGGGCTGAGAGCCACTGAATGC2570.10057488132555346No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCTC100.0070882807144.99669145
AGCGAAA1950.0107.8813861
GAGCGAA501.9281288E-10101.55731
CAGGACG650.0100.441284
GTCCTGG850.093.876491
GCAGGAC700.093.26693
GACGGTG800.090.6229257
ACGGTGA801.8189894E-1281.560638
GCGAAAG2600.080.911032
CGAAAGC2750.076.49773
GAAGGGA702.7885108E-972.540924
GAAAGCA2950.071.311424
AAGACTT808.056304E-963.436047145
CAGGTGT350.003563790362.141439
AGCAGGC1550.060.8407753
GCAGGAG1251.8189894E-1258.032741
ACCTGAG1007.1304385E-1058.0327383
GGGGTTG753.7354584E-758.0327385
TACCTGA1007.1304385E-1058.0327382
GGGTTGA753.7405152E-758.0213786
Produced by FastQC (version 0.11.5)