FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9567_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9567_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041848
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACG146920.7195442559877131No Hit
TTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCC121370.5944125125866372No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT112880.5528325320983737No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC93260.4567431072244359No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG89870.4401405001743518No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG87840.43019852604111564No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT79340.38856957031081646No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG72370.3544338266119711No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT69410.33993715496941984No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC68200.3340111506831067No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA67950.33278676963221554No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG64420.3154985091936324No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC61560.30149158997143766No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA57260.28043223589610977No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC56440.27641626604918684No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC54630.2675517472407349No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA53150.26030341141945923No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT52280.256042565362358No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT49530.2425743738025553No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA47820.23419960741445983No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA46860.22949798417903783No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG46190.22621664296264954No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT45900.22479636094361577No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC43150.2113281693838131No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT41400.202757502027575No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA39390.19291347837841016No Hit
TGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCA39310.19252167644212498No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG38730.18968111240405752No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG38510.1886036570792733No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC38080.1864977216717405No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC37300.1826776527929601No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC37180.18208994988853233No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT36050.17655574753850434No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC36030.17645779705443304No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT35930.1759680446340766No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT35330.1730295301119378No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG35270.17273567865972395No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC35260.1726867034176883No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG34570.1693074117172287No Hit
GTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT34430.16862175832872967No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT34350.16822995639244448No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC34040.16671172388933944No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT34020.16661377340526817No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA33690.16499759041809184No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT33200.1625978035583452No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA33040.16181419968577485No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG32930.16127547202338274No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG32090.15716155169238846No Hit
GACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCT31830.15588819539946167No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA31760.15554536870521213No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA31550.15451688862246357No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT31200.15280275515121597No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT31130.15245992845696643No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT30610.14991321587111284No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA30450.1491296119985425No Hit
GTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG30330.14854190909411474No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA30270.14824805764190085No Hit
CTTAAAATGACCATGGTCTCAACACCTGCTTCGAGATACATAACTGACAT30260.1481990823998652No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA29620.14506466690958386No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT28510.13962841504362716No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACTTGAATCGTTGCA28150.13786530633034388No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA27850.1363960490692745No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT27800.13615117285909625No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG27600.13517166801838335No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCACT27590.1351226927763477No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA27400.13419216317767044No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC27170.13306573261085058No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC26980.1321352030121733No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA26950.13198827728606635No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG26880.13164545059181681No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA26850.1314985248657099No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA25700.12586637203161058No Hit
ATACATAACTGACATGACTATTGAAGAATTGTCAAGAAACTGGTTCATGC25660.125670471063468No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC25520.12498481767496895No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC25490.12483789194886201No Hit
CTATTGAAGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA25170.12327068420372132No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT25150.12317273371965005No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG25140.1231237584776144No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG25120.1230258079935431No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC24940.12214425363690148No Hit
GTCTAGACATCAAATCAGCCACCCATGTTGGAAAGCAAATTGTAGAAAAG24480.11989139250326175No Hit
GTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAG24460.11979344201919045No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT24340.11920573911476272No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC24280.11891188766254882No Hit
GTAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT24190.11847111048422802No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCGTGGAACGTTATCTCCCTCTT23930.11719775419130121No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG23900.11705082846519427No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCA23360.11440616539526939No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTG23080.11303485861827128No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT22950.11239818047180788No Hit
GTTCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGCCTATGAG22710.11122277466295237No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA22700.11117379942091675No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG22680.11107584893684545No Hit
ACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTCACCAT22670.1110268736948098No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACAT22640.11087994796870285No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC22250.10896991352931266No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG21940.10745168102620764No Hit
AACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAAC21910.1073047553001007No Hit
CCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTT21870.10710885433195812No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG21750.10652115142753035No Hit
CCCCCATTCGTTTCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTT21550.10554164658681743No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC21390.10475804271424709No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT21280.104219315051855No Hit
GACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACG21230.10397443884167674No Hit
TATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACC21070.10319083496910642No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA21030.10299493400096384No Hit
CAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGTTCCTTTCGATAT20930.10250518158060737No Hit
GGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAG20930.10250518158060737No Hit
CCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG20900.10235825585450044No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG20690.10132977577175187No Hit
CTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAGCTCTCAGTTA20640.10108489956157363No Hit
GGTCTCAACACCTGCTTCGAGATACATAACTGACATGACTATTGAAGAAT20590.1008400233513954No Hit
ACATAACTGACATGACTATTGAAGAATTGTCAAGAAACTGGTTCATGCTA20480.10030129568900328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCG203.8667876E-4108.748955145
TATTAGT451.08957465E-896.7012561
GGTAGTC451.2334185E-680.554788
TGTAAGG300.001934413872.515283
ACTAATA1000.072.4993068
AGCGAAA7750.072.058041
GCGAAAG8250.068.5599062
GAGCGAA2900.067.5241551
CTACGCA554.0633513E-665.908455145
CGAAAGC8600.065.769683
CAGTACG2500.063.7993859
CTACTAA1150.063.0536846
AGTAGAA12750.060.8649061
TTAGTAG1200.060.42942
AGGCGCT606.8085155E-660.4160847
GTTGATA1450.055.0196841
CCTACCC400.00605201554.374477145
TACTAAT1353.6379788E-1253.703197
CACTGTG33100.053.6626247
GGGTCAT953.1326636E-853.4401661
Produced by FastQC (version 0.11.5)