FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9504_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9504_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497936
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAA42580.2842578054069066No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG41000.2737099582358659No Hit
CAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAGCAATGTG33590.22424189017421306No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29520.19707116992982343No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT29230.19513517266425268No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA26630.17777795580051484No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26100.17423975390136828No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA24400.1628908044135397No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA22490.1501399258713323No Hit
GTAGAAACAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAG21100.14086049070187243No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACCAAAAATCTCAATT20010.13358381132438235No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19250.12851016331805898No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT18910.12624037342049327No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC18860.1259065807884983No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18750.1251722369981094No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA18720.12497196141891243No Hit
AGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGACAGAT18030.120365623097382No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT17630.11769528204142234No Hit
AACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGAC17430.1163601115134425No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17370.11595956035504855No Hit
GCTAATGGAGTGACCACACATTATGTTTCTCAGATTGGCGACTTCCCAGA17250.11515845803826065No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17110.11422383866867476No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA16940.11308894371989192No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16930.11302218519349291No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC16560.11055211971673022No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA16410.10955074182074534No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA16130.10768150308157359No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC16100.1074812275023766No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT15900.10614605697439677No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC15810.10554523023680584No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15690.10474412792001796No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT15620.104276818235225No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA15560.10387626707683106No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC15530.10367599149763407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCAT950.0106.865883
CGGTGCA1000.0101.522582
GCACGCA2700.083.2592542
AGCACGC2750.081.767291
AACACGT4300.079.259166
CACGCAC2850.078.877193
ACACGTG4450.076.572177
AAACACG10650.074.218385
GAAACAC11350.069.002134
ACGTGCA3300.068.105299
GCGGTGC2450.068.094551
AGCGTTT451.192738E-464.458785
CGGTGCC702.3317443E-762.1566772
CGAGCAC350.00356370962.1566773
AACACGA7550.059.5476766
AACAACG5350.058.270377
ACACGAG7750.057.9993447
CACGTGC5950.057.268268
TAGTAAC31300.056.9935042
ACAACGC5350.056.9152458
Produced by FastQC (version 0.11.5)