FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9504_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9504_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506916
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAA42830.28422287639125204No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG41300.27406968935229303No Hit
CAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAGCAATGTG33760.22403372185310927No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29660.1968258350166831No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT29330.19463593192984877No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA26820.17797939632998785No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26200.17386503295472341No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA24470.16238463192374358No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA22620.1501079024975513No Hit
GTAGAAACAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAG21170.14048560105540056No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACCAAAAATCTCAATT20150.13371680969609454No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19330.1282752323288093No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT19030.1262844113407781No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC18920.12555444364516669No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18880.12528900084676253No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA18860.12515627944756047No Hit
AGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGACAGAT18110.12017922697748248No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT17750.11779024179184507No Hit
AACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGAC17470.11593214220301595No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17420.11560033870501077No Hit
GCTAATGGAGTGACCACACATTATGTTTCTCAGATTGGCGACTTCCCAGA17380.11533489590660662No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17210.11420676401338893No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA17010.11287955002136814No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT17000.11281318932176712No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC16610.11022512203732657No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA16480.10936243294251305No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA16240.10776977615208812No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC16230.10770341545248707No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT16010.1062434800612642No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC15890.10544715166605173No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15750.10451810187163717No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT15720.10431901977283405No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA15630.10372177347642471No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC15610.10358905207722262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACG100.007085827145.036873
CGCACGT100.007085827145.036874
CGCACCC203.8666138E-4108.74878145
GGTGCAT800.099.712853
CGGTGCA950.091.6022342
AGCACGC3100.091.25711
CACGCAC3100.091.232873
GCACGCA3100.091.232872
ACAACGC5850.071.879548
GCGGTGC2250.070.925741
CAACGCA6200.067.821829
GGAGCGT554.0564755E-665.925853
CGGAGCG554.0564755E-665.925852
GAGCGTT702.331235E-762.158664
CGGTGCC702.331235E-762.158662
AGCGTTT702.3328357E-762.1524735
CAGCGAC350.003567012562.142162145
ACGTGCT1051.6370905E-1162.142169
AACAACG6900.061.992067
AAACACG11950.060.0720525
Produced by FastQC (version 0.11.5)