FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9497_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9497_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424848
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATTT69470.4875607784128553No Hit
GAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATT45070.3163144419615285No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT44440.3118929177006951No Hit
GTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAGGCACT40750.28599541845867066No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT39440.2768014553131281No Hit
GTGCATACTCTCGGGGCGGTGACTGCTGTGAGCACGTGCGATCTCGGATG38640.27118682133111743No Hit
GGTATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC37600.2638877971545035No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA37160.2607997484643976No Hit
TCTCACGACAACTCCCGCCTCGACAAATATTAAAGCGCTTCGCTTCCGAA36170.25385163891165935No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31200.21897072529841777No Hit
CCCCGAGAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACA30770.21595285953308704No Hit
CTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCTGTCCGTAGAGGT28890.20275846967536185No Hit
CACGTGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC28700.20142499410463433No Hit
GTCTACGAGGCACTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCT27140.19047645783971343No Hit
ATATTAAAGCGCTTCGCTTCCGAACAGACATAAAATGGACACGCAAGTCG25860.1814930434684963No Hit
GTCCGTAGAGGTCCCACTTGCAGATGAGTACGGCCAAGGCCTTGCTTGGA25070.1759485924112607No Hit
TATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGACTG24150.16949176333194838No Hit
GCATAAAGGCGGGTGTCCTGCTTTCTTATTTCATCGTTGTCCCACTCTCC23790.16696517804004357No Hit
GACATAAAATGGACACGCAAGTCGGGTCAGCCCCGACCGCCTTAACGATT23770.1668248121904933No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22900.16071889773505665No Hit
CTTTTTTGGTGCACCTCTCACGACAACTCCCGCCTCGACAAATATTAAAG22830.16022761726163073No Hit
GTAGTAACAAGAGCCGGGAAAGGTTGCATAAAGGCGGGTGTCCTGCTTTC22150.1554551783769216No Hit
TTTCATGTCTGTTCGGAAGCGAAGCGCTTTAATATTTGTCGAGGCGGGAG20880.14654194693047962No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20410.1432433494660483No Hit
CACGAGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC20390.14310298361649804No Hit
GAGTAAGGCCACTCAATGACATCCATTTCCCAGGTATATGCCATCTACAC20360.14289243484217265No Hit
GGCATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC19980.14022548370071755No Hit
GTTCACAGGAGACTGGGAAGAATATCCCGTACGAAACCCTTTACCCGAAG19820.13910255690431542No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT19550.1372076179353868No Hit
CTTTAATATTTGTCGAGGCGGGAGTTGTCGTGAGAGGTGCACCAAAAAAG19230.1349617643425825No Hit
CGTATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGAC19090.13397920339573063No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC18600.13054024008174908No Hit
GCCTTGTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAG18030.12653981336956643No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17900.1256274353474897No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17620.12366231345378595No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA17390.12204810618395787No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC16990.11924078919295251No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT16950.11896005749385198No Hit
CGTCTAAACAGATCGAATACGTATATATGCCGGGTCATTGCAAAAACGGA16110.11306469181274073No Hit
GCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGACCGAGT15990.11222249671543913No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC15990.11222249671543913No Hit
CTCTCCACCCTGAGCTTTTCTTAGTGTTTTAAGCGTGGGTTGGAGAGCGA15950.11194176501633857No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT15550.10913444802533323No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG15450.10843261877758188No Hit
GAATATCCCGTACGAAACCCTTTACCCGAAGACATCCGAGATCGCACGTG15110.10604639933522735No Hit
GTTTAGACGCGAAAATTGTTCACGCTCTGCCGAGGCGCTTACGGCCAAGG14750.10351981404332251No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT14610.10253725309647063No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14600.1024670701716955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCGA100.00709155144.99745145
CTAACTT203.8601956E-4108.79394
TGGGGTC301.597048E-596.69895
AGCACGC5500.094.9740751
GCACGCA5450.094.487662
GGGGTCA406.656633E-572.516536
AGCCCGC300.001936047272.49872145
TCTAACT350.00356111762.167943
GAGCGCC400.006041757854.396954
GCGCCGG400.00604595554.38746
ACGTGCA3750.052.221072
ACAGCAT2000.050.770484
GAGCTCA7350.050.3264274
GTAAGAA11700.048.986511
GATCCAT450.00961393848.366431
CACGCAC10800.047.6812743
GTAACAA28200.046.2952734
CGAGCTC8150.046.2763373
GGTGCGT1002.7273818E-643.5175553
TAGTAAC31250.042.9373252
Produced by FastQC (version 0.11.5)