FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9497_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9497_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433504
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATTT69870.4874070808313057No Hit
GAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATT45260.3157298479808916No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT44670.31161405897716365No Hit
GTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAGGCACT40990.2859426970556064No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT39690.2768740094202737No Hit
GTGCATACTCTCGGGGCGGTGACTGCTGTGAGCACGTGCGATCTCGGATG38780.27052592807554077No Hit
GGTATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC37820.26382905105252585No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA37420.26103868562626964No Hit
TCTCACGACAACTCCCGCCTCGACAAATATTAAAGCGCTTCGCTTCCGAA36400.25392325378931624No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31420.2191832042324263No Hit
CCCCGAGAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACA30970.21604404312788802No Hit
CTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCTGTCCGTAGAGGT29030.20251077081054536No Hit
CACGTGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC28850.20125510636873006No Hit
GTCTACGAGGCACTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCT27260.19016340379936156No Hit
ATATTAAAGCGCTTCGCTTCCGAACAGACATAAAATGGACACGCAAGTCG25940.18095519789271602No Hit
GTCCGTAGAGGTCCCACTTGCAGATGAGTACGGCCAAGGCCTTGCTTGGA25130.17530470790454716No Hit
TATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGACTG24300.16951469964506552No Hit
GACATAAAATGGACACGCAAGTCGGGTCAGCCCCGACCGCCTTAACGATT23980.16728240730406052No Hit
GCATAAAGGCGGGTGTCCTGCTTTCTTATTTCATCGTTGTCCCACTCTCC23930.16693361162577852No Hit
CTTTTTTGGTGCACCTCTCACGACAACTCCCGCCTCGACAAATATTAAAG22960.16016697546710718No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22940.16002745719579436No Hit
GTAGTAACAAGAGCCGGGAAAGGTTGCATAAAGGCGGGTGTCCTGCTTTC22240.15514431769984596No Hit
TTTCATGTCTGTTCGGAAGCGAAGCGCTTTAATATTTGTCGAGGCGGGAG20970.14628490747148246No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA20550.14335502377391343No Hit
CACGAGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC20510.1430759872312878No Hit
GAGTAAGGCCACTCAATGACATCCATTTCCCAGGTATATGCCATCTACAC20460.14272719155300578No Hit
GGCATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC20070.14000658526240597No Hit
GTTCACAGGAGACTGGGAAGAATATCCCGTACGAAACCCTTTACCCGAAG19980.1393787530414983No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT19620.1368674241578677No Hit
CTTTAATATTTGTCGAGGCGGGAGTTGTCGTGAGAGGTGCACCAAAAAAG19320.13477465008817555No Hit
CGTATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGAC19190.13386778132464228No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC18710.13051934281313482No Hit
GCCTTGTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAG18150.1266128312163761No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT18070.12605475813112485No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17690.12340391097618143No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA17450.1217296917204277No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT17100.1192881219724535No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC17060.11900908542982788No Hit
CGTCTAAACAGATCGAATACGTATATATGCCGGGTCATTGCAAAAACGGA16170.11280052235640779No Hit
CTCTCCACCCTGAGCTTTTCTTAGTGTTTTAAGCGTGGGTTGGAGAGCGA16060.11203317186418732No Hit
GCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGACCGAGT16050.11196341272853093No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC16040.11189365359287452No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT15620.10896376989530548No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG15490.10805690113177222No Hit
GAATATCCCGTACGAAACCCTTTACCCGAAGACATCCGAGATCGCACGTG15220.10617340446904926No Hit
GTTTAGACGCGAAAATTGTTCACGCTCTGCCGAGGCGCTTACGGCCAAGG14800.10324352077148023No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14720.10268544768622899No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT14680.10240641114360337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG100.007091323144.999028
TCAACGT100.007091323144.999029
AGCACGC6100.085.600071
GCACGCA6400.082.70632
GATCCAT300.001933556272.522281
CAGCTCG300.001934894772.509635
GCCGATA300.001935966172.49951145
ATGGATC554.0593004E-665.917853
GCACGCT350.00356494362.151114
GAGCTCA7250.057.0075724
CGAGCTC8050.052.242963
GTAACAA29200.048.4225234
TAACACG604.942567E-448.3397525
ACAGCCG1351.9099389E-1048.3397524
AGCCACA450.00963625548.3380666
GCACTTT14350.047.4959376
GTAAGAA12400.047.3734251
CACGCAC11500.046.027853
CCCTGCT803.7356178E-545.3121957
TAGTAAC33500.043.5057752
Produced by FastQC (version 0.11.5)