FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9464_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9464_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856398
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC104650.5637260975286549No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG70650.3805757170606734No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT47570.2562489293782907No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG46870.25247818625100865No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG45730.24633726172943515No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC44440.23938832082344413No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC43130.2323316443995307No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG38820.209114640287266No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC38430.2070137976877803No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG34620.18649018152357416No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG33850.18234236408356397No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAGACACAGGGAACTGAAAGACT33530.1806185957968065No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT31710.1708146636658734No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA31160.16785193692300898No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG30310.16327317741130942No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG30260.16300383861650358No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATAGGCTTCG29420.1584789468637652No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA29270.15767093047934763No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT29210.1573477239255806No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA27790.14969850215309433No Hit
GTTGAGACCATGGTCATTTTAAGTGTCTCATCAGATTCTCCTTTCAGAAT27700.1492136923224438No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT27390.1475437917946475No Hit
CTCCAGTACAGAGAGGGTGGTGGTTAGCATTGATCGGTTTTTGAGAGTTC27310.14711284972295813No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG26860.14468880056970543No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC26410.1422647514164527No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA26310.14172607382684102No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC26070.14043324761177292No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTAACTCTTATTCCTCTCAT25670.13827853725332606No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA24130.1299829023733057No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT24010.12933648926577168No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC23800.12820526632758708No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC23280.1254041428616062No Hit
CTCCTGAGGAGGTCAGTGAGACACAGGGAACTGAAAGACTGACAATAACT22390.12060991231406196No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT22230.11974802817068322No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA22160.11937095385795504No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA21980.11840133419665395No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT21980.11840133419665395No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA21890.1179165243660034No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC21710.11694690470470234No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC21450.11554634297171189No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT21330.11489992986417784No Hit
TTCTTAAACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT21160.11398417796183793No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT20770.11188333536235225No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC20630.11112918673689587No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT20560.11075211242416766No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA20450.1101595670755948No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT20120.10838193102987613No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC20110.10832806327091496No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA19810.10671203050207984No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT19670.10595788187662343No Hit
CTCTTATTCCTCTCATTGACATCTCTGTACTTGGAGTCATATCTGGTAAT19630.10574241084077875No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA19590.10552693980493406No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG19560.10536533652805057No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA19550.10531146876908938No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19510.1050959977332447No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCGATTTGTACTCCTCTAGTT19360.10428798134882714No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT19240.10364156824129309No Hit
ATATGGGATCAAGCACTCTTGAACTGAGAAGCGGATACTGGGCCATAAGG19030.1025103453031085No Hit
GGTGGCTTTACATTTAAAAGAACAAGCGGGTCGTCAGTCAAAAAAGAAGA18970.10218713874934146No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT18880.10170232891869094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA100.0070869764145.02992
CCTCGCC151.230219E-4144.99866145
TCGACCA100.007091544144.998669
AGCGAAA4350.0105.0329741
GCCTGCC651.6370905E-1189.22994145
CGAAAGC5250.087.0179443
GAGCGAA1200.078.566331
TTATATT3350.069.268012
GCAGGGG554.0631985E-665.908478
AATTCTA1700.063.983785
TATATTC3700.062.7156333
ATATTCA3600.062.4434284
GCTGACC350.00356711662.142277145
TTCTATT1800.060.4161037
GGGTGAC8350.059.060461
CAGGGTG4350.058.332799
GTATTAG1500.058.018211
TACTGAT1500.057.999467
GTACTGA1550.056.137586
GTATTAA9950.054.6653981
Produced by FastQC (version 0.11.5)