FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9443_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9443_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231022
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG9380.40602193730467234No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA9110.39433473868289604No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT8830.3822146808529058No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT7090.3068971786236808No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT6200.2683727090926405No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA6060.2623126801776454No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT5710.24716260789015765No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA5280.2285496619369584No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC5240.22681822510410266No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC5240.22681822510410266No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG5010.2168624633151821No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT4870.210802434400187No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA4870.210802434400187No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG4860.21036957519197305No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG4600.1991152357784107No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT4540.19651808052912711No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA4360.18872661478127623No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4350.1882937555730623No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT4180.1809351490334254No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA4060.17574083853485817No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA3860.16708365437057943No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA3860.16708365437057943No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA3760.16275506228844008No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG3650.15799361099808676No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT3640.1575607517898728No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT3570.15453073733237527No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA3420.1480378492091662No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG3400.14717213079273836No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT3390.14673927158452443No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT3330.1441421163352408No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA3320.14370925712702687No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG3320.14370925712702687No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT3150.13635065058738993No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG3110.1346192137545342No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT3100.13418635454632025No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA3090.13375349533810635No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC3020.13072348088060876No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC3010.13029062167239483No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA2920.1263948887984694No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG2910.1259620295902555No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG2780.1203348598834743No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG2700.1168719862177628No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA2700.1168719862177628No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA2700.1168719862177628No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC2680.11600626780133494No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT2660.11514054938490707No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2640.1142748309684792No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA2620.11340911255205133No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT2590.11211053492740952No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT2590.11211053492740952No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT2580.11167767571919557No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT2580.11167767571919557No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG2560.1108119573027677No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT2490.10778194284527015No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT2490.10778194284527015No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2470.10691622442884229No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA2450.10605050601241439No Hit
GCTAATGGAGTGACCACACATTATGTTTCTCAGATTGGCGACTTCCCAGA2420.1047519283877726No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG2410.10431906917955866No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT2370.10258763234670291No Hit
CTCCATTAGCAGATGAGGTGAACTTTTGAGGATTTGAGTCTCCATAGAGG2350.10172191393027503No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC2350.10172191393027503No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT2340.10128905472206111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC700.0134.728131
GCACGCA700.0134.698962
CACGCAC750.0125.719033
ACGCACT1200.078.5743944
CGCACTT1200.078.5743945
GTCATAA406.63151E-572.545921
AGAAGCG300.001930657472.530213
CCGAGTC300.001933977672.49881145
GAAGCGG451.1881926E-464.485261
GTTTCTA808.00901E-963.4776761
AAGCGGA350.003557155862.1687552
AAACAAC502.007158E-457.9990466
ACAACGC502.007158E-457.9990468
GTATAAA400.006029892754.409441
AGCGGAG805.8282967E-754.409441
AACACGA400.006029892754.409441
TAAATAG400.006035062554.397664
AAATTCG400.006045412354.3741079
TACTGGG400.006045412354.3741078
TAGTAAC2900.052.5218732
Produced by FastQC (version 0.11.5)