FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9420_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9420_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962845
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC128700.6556809121453808No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA83730.4265746913281487No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG69480.35397598893442933No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG64210.32712720566320824No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT59740.30435413901759945No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA54180.27602790846959385No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC50360.25656636158229507No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC50160.255547432425892No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC47680.24291271088649385No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT47630.24265797859739308No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC47090.2399068698751048No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC44820.22834202394992983No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG43690.22258507421625243No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC43340.22080194819254703No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA39860.2030725808711335No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA39520.20134040130524827No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC39400.20072904381140644No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT39370.2005762044379459No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT39020.19879307841424057No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA37090.18896041205495084No Hit
CACTTGGAGGTGTGTTTCATGTATTCAAGTCAGTATTCAATAGCCTGTAT35570.18121655046628746No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG31770.16185689649462898No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG31150.1586982161097794No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA30920.15752644757991588No Hit
ATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA30130.15350167741212373No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG29680.1512090868102168No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA29500.15029205056945402No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA29360.14957880015997188No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC28270.1440256362575751No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA28250.14392374334193478No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT27910.14219156377604955No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA27860.1419368314869488No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG27780.14152925982438755No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT27740.14132547399310694No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT27630.14076506295708527No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG27190.13852341881299848No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT26910.13709691799403417No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC26900.137045971536214No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC26460.13480432739212725No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA26410.13454959510302647No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG25520.13001536035703276No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA24980.12726425163474447No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT24930.1270095193456437No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC24870.12670384059872275No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT24570.12517544686411816No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA24020.1223733916840097No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA23860.12155824835888723No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA23780.12115067669632601No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG22970.11702401361289352No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG22460.11442574426406568No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT21970.11192936783087815No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA21810.11111422450575568No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT21260.1083121693256472No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT21200.10800649057872629No Hit
GTAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT21170.10785365120526583No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC21070.1073441866270643No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT20760.10576484643463951No Hit
CTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCA20700.1054591676877186No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA20390.10387982749529383No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA20240.10311563062799152No Hit
GTCTAGACATCAAATCAGCCACCCATGTTGGAAAGCAAATCGTAGAAAAG20130.10255521959196981No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC19840.10107777231518536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6400.095.20321
CTGGACG700.093.253143
CGGGAGC700.093.212768
CGAAAGC7950.076.635713
ATAAGTG702.8048817E-972.530223
TATATTC5300.069.7932363
TAAACTA3650.069.542445
GACGGGA955.456968E-1268.698846
AATTCTA1700.068.256785
GTACTGA8650.067.904636
GGACGGG1009.094947E-1265.2705465
ATATTCA5700.064.895464
TAGAAAC14750.062.9414862
CAGTACG15550.062.9410939
CCTACCC350.003561130562.1687623
GTTAGGC350.003561130562.1687623
TACTGAT9700.060.540257
GAGCGAA2900.060.0296061
ACGGGAG1102.5465852E-1159.317217
CACTGTG33500.059.0811237
Produced by FastQC (version 0.11.5)