FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9332_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9332_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227565
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGTAGTGGTCGTAATTAGTTTGTATGTGCTTCTGTCATTTTGCTCA44040.35875900665137894No Hit
GATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGA43770.3565595304525626No Hit
GTCCTGGATCTCTGACCATGTTTCTGATGAACGAAGGACCGACACTTGGG42340.3449104528069797No Hit
AGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTC42270.3443402182369162No Hit
CATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCTGG38870.3166431105481176No Hit
GGTTTAAGATAAGGCCCCCGCCCGCATGAATTTTTTATCCAGACAAGGCA34370.27998517390117833No Hit
GCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGAGAGAGAGACA33710.27460867652629395No Hit
GTATTTAATGAGGGATTTTTAATGAATTAATAAAAGAGAGACGTGTTGTA33710.27460867652629395No Hit
GTATAATAAAGATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTA30840.2512290591536904No Hit
GTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTG30740.250414438339314No Hit
CACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCT29010.2363214982506018No Hit
AAACAAGGACGAGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAA23610.19233197427427468No Hit
GTACATGCATGTCCATGTCATACATAACCATAATTGACACCGGGAAAAAC23200.18899202893533132No Hit
GCCTTAGTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCG22970.18711840106226554No Hit
GTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGA21830.17783172377837425No Hit
CCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAGCTTATTCCATTA21800.17758733753406133No Hit
GTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTAGTTTGTATGT21580.1757951717424332No Hit
ATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTG21170.17245522640348984No Hit
TAGTTATAGTTATTAGTTATTTTTGCAACTTTTGTAAGACTCACGTTACT20900.17025575020467348No Hit
GTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTT19930.16235392830522213No Hit
GTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGATCTATTTACCCT19570.159421293373467No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC18750.1527414026955803No Hit
CTTTTTGACTGGTCACTGTATTTAATGAGGGATTTTTAATGAATTAATAA18740.15265994061414262No Hit
ATATTAAATTCTCCCTCCAGTCGACACCACCCGTGTGATCGTCAGGGAAG18440.15021607817101335No Hit
GTAGTAATAAGTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTA18410.14997169192670043No Hit
GTTTAAACACCGGGTGGTGCTGTGGGTCCACGTTTAGCTCCCCCTTGGTC18130.1476907536464464No Hit
CTGTTTACACTTCCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAG17530.14280302876018786No Hit
AGTGAATGGTGTGCCCTCTCCGCTCTGTGTCCACAGATGCTGCAGCAGCA17420.14190694586437377No Hit
CTATTTACCCTGTTGCCAACTCAGTACATGCATGTCCATGTCATACATAA16940.13799676595536692No Hit
GAACACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGG16410.13367927563917187No Hit
GTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGGTGTCAATT16120.1313168752774802No Hit
CCCTGCATCTGCGCCACAGAGACGACTGCCACCCTGCATCTGCGCCACAG15940.12985055781160265No Hit
GTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCGCATATC15920.12968763364872735No Hit
GTTTAGTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTG15490.1261847641469087No Hit
GGAATGAACAATGTCAATGATTTGACGGGGAAAACTCACTCTGATGTAGT15350.1250442950067817No Hit
TACCTGGAGTGAACACTCAGCAAGGGCCAAAGAGAACCTCAAAACACTGA15080.12284481880796537No Hit
GGTACAGGGAGGGCAGATGGTAGAGGTGCAGGGGGATCAGCAGCACAGTC14870.12113411509777486No Hit
GATGCAGGGTGGCAGTCGTCTCTGTGGCGCAGATGCAGGGTGGCAGTCGT14200.1156761556414528No Hit
GTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTCAATGA13590.1107069686737566No Hit
CTCTAGTTGTGTGCACGTATCCTAATTGTCATTTTGCAAATTAGTCATCG13440.10948503745219194No Hit
GTGTCCACAGATGCTGCAGCAGCAGGAGTGCAAGCTCCTCTACCCTAGGA13440.10948503745219194No Hit
GGCTCAGGTTGTGCATGGGTCTCAATTTTTTGTGGAAGCACAGACCTTAC13270.10810018206775202No Hit
GCATATCACATATCACATACGATTGGAAAAAATATTTTACACTAATCTCA12990.105819243787498No Hit
CTCCAGGCCTGTTCCTGGTGAGCAGCGGCCAGGCAGCCACCGGGGGGTTG12960.10557485754318509No Hit
CATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCAC12950.10549339546174744No Hit
ATGATGAAGGGCCCGAGCCGATGCTCTATGAAGTTTAAACACCGGGTGGT12880.10492316089168394No Hit
GAGTAAGGAGTGTGCTGCAACAAGCTCATACCTGGAGTGAACACTCAGCA12710.10353830550724401No Hit
GTGTTGTAGTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGG12360.10068713265692653No Hit
CCTTACTCCCTCCAACTCCCTGTACTCCACCCTGGGGAAAAACCTCAAGC12300.10019836016830065No Hit
ATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCACT12290.10011689808686303No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG151.2285443E-4145.045273
CTACGGG151.2285443E-4145.045274
CGGCTCC203.8664803E-4108.748505145
AGCGAAA12650.0103.211081
TACGGGC259.377146E-487.023615
GCGAAAG15300.085.7947542
GAAAGCA17400.075.857014
GTATAGG1400.072.5344541
TATAGGC1450.070.021852
GAGCGAA3650.069.553591
CGAAAGC18900.069.452893
ATAGGCC1500.067.687793
GCCTGTA1500.067.665747
CTTAGAC651.3953104E-766.943973
GGTAGCG350.003566890762.142002145
TAGTGCA1350.059.0925184
AAGGACG6050.057.5362745
CGTGACT400.00605154254.3742527
TCTTAGA953.1324817E-853.437732
GTCTTAG553.2117474E-452.752331
Produced by FastQC (version 0.11.5)