FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9332_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9332_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235491
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGTAGTGGTCGTAATTAGTTTGTATGTGCTTCTGTCATTTTGCTCA44260.35823814175902535No Hit
GATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGA43940.355648078375318No Hit
GTCCTGGATCTCTGACCATGTTTCTGATGAACGAAGGACCGACACTTGGG42660.34528782484048853No Hit
AGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTC42470.3437499747064122No Hit
CATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCTGG39110.3165543091774849No Hit
GGTTTAAGATAAGGCCCCCGCCCGCATGAATTTTTTATCCAGACAAGGCA34510.2793221480366915No Hit
GTATTTAATGAGGGATTTTTAATGAATTAATAAAAGAGAGACGTGTTGTA33910.27446577919224013No Hit
GCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGAGAGAGAGACA33860.2740610817885359No Hit
GTATAATAAAGATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTA31010.25099332977739214No Hit
GTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTG30960.25058863237368784No Hit
CACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCT29170.2361004653210748No Hit
AAACAAGGACGAGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAA23770.19239314572101293No Hit
GTACATGCATGTCCATGTCATACATAACCATAATTGACACCGGGAAAAAC23300.18858899012619273No Hit
GCCTTAGTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCG23070.18672738206915307No Hit
GTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGA21950.17766216022617728No Hit
CCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAGCTTATTCCATTA21880.17709558386099128No Hit
GTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTAGTTTGTATGT21680.17547679424617418No Hit
ATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTG21300.1724010939780217No Hit
TAGTTATAGTTATTAGTTATTTTTGCAACTTTTGTAAGACTCACGTTACT20980.16981103059431432No Hit
GTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTT20040.16220271940467393No Hit
GTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGATCTATTTACCCT19710.15953171654022572No Hit
CTTTTTGACTGGTCACTGTATTTAATGAGGGATTTTTAATGAATTAATAA18880.1528137396387347No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC18820.1523281027542896No Hit
ATATTAAATTCTCCCTCCAGTCGACACCACCCGTGTGATCGTCAGGGAAG18510.1498189788513231No Hit
GTAGTAATAAGTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTA18510.1498189788513231No Hit
GTTTAAACACCGGGTGGTGCTGTGGGTCCACGTTTAGCTCCCCCTTGGTC18170.147067036506134No Hit
CTGTTTACACTTCCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAG17670.14302006246909124No Hit
AGTGAATGGTGTGCCCTCTCCGCTCTGTGTCCACAGATGCTGCAGCAGCA17510.14172503077723755No Hit
CTATTTACCCTGTTGCCAACTCAGTACATGCATGTCCATGTCATACATAA17000.13759711725945392No Hit
GAACACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGG16510.13363108270315205No Hit
GTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGGTGTCAATT16280.13176947464611236No Hit
GTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCGCATATC16000.12950316918536842No Hit
CCCTGCATCTGCGCCACAGAGACGACTGCCACCCTGCATCTGCGCCACAG15970.12926035074314585No Hit
GTTTAGTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTG15570.12602277151351163No Hit
GGAATGAACAATGTCAATGATTTGACGGGGAAAACTCACTCTGATGTAGT15470.12521337670610308No Hit
TACCTGGAGTGAACACTCAGCAAGGGCCAAAGAGAACCTCAAAACACTGA15170.12278519228387742No Hit
GGTACAGGGAGGGCAGATGGTAGAGGTGCAGGGGGATCAGCAGCACAGTC14990.12132828163054202No Hit
GATGCAGGGTGGCAGTCGTCTCTGTGGCGCAGATGCAGGGTGGCAGTCGT14300.11574345745942302No Hit
GTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTCAATGA13690.11080614913423084No Hit
GTGTCCACAGATGCTGCAGCAGCAGGAGTGCAAGCTCCTCTACCCTAGGA13550.10967299640385886No Hit
CTCTAGTTGTGTGCACGTATCCTAATTGTCATTTTGCAAATTAGTCATCG13510.10934923848089544No Hit
GGCTCAGGTTGTGCATGGGTCTCAATTTTTTGTGGAAGCACAGACCTTAC13310.10773044886607834No Hit
CTCCAGGCCTGTTCCTGGTGAGCAGCGGCCAGGCAGCCACCGGGGGGTTG13040.10554508288607525No Hit
GCATATCACATATCACATACGATTGGAAAAAATATTTTACACTAATCTCA13040.10554508288607525No Hit
CATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCAC12990.10514038548237097No Hit
ATGATGAAGGGCCCGAGCCGATGCTCTATGAAGTTTAAACACCGGGTGGT12920.10457380911718499No Hit
GAGTAAGGAGTGTGCTGCAACAAGCTCATACCTGGAGTGAACACTCAGCA12740.1031168984638496No Hit
GTGTTGTAGTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGG12480.10101247196458735No Hit
ATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCACT12360.1000411981956971No Hit
CCTTACTCCCTCCAACTCCCTGTACTCCACCCTGGGGAAAAACCTCAAGC12360.1000411981956971No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG203.8645227E-4108.762396
AGCGAAA13350.0108.652991
GCGAAAG16600.087.362882
TACCGGT259.387557E-486.9993447
GAAAGCA18650.077.7599954
CGAAAGC20300.071.796793
CACTAGA851.6916601E-1068.245832
GAGCGAA3350.062.7835921
AAGGTCC954.5656634E-1061.0595865
AAGGACG5850.059.493965
ACCACTC1007.203198E-1057.999568
TAGTGCA1600.054.38344
ACGAGTA6650.052.3304379
CATATGG11300.051.987571
TAGAAAC7650.051.184372
AGTAGGT12100.050.348541
TCTAGTG3900.050.2000582
CTAGTGC4000.048.945053
TAGGTAC12600.048.3407973
CCAGATC450.00963379448.3407944
Produced by FastQC (version 0.11.5)