FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9320_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9320_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915861
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA66230.345693137445775No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA52350.2732452928474456No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT52010.27147063382990727No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT51610.2693827996916269No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA48610.253724043654524No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG45370.23681258713445286No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT43510.22710415839144907No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT39090.20403359116345077No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA37710.19683056338638347No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT37670.19662177997255542No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG36630.1911934112130264No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG33340.17402097542567024No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT32990.1721941205546749No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC32820.17130679104590574No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC30920.1613895788890739No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT30760.16055444523376175No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC30670.16008468255264865No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG30510.1592495488973365No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA29980.156483168664115No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC27270.14233809237726536No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26100.13623117752279523No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT26080.13612678581588122No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25800.13466530191908493No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT25130.1311681797374653No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24830.129602304133755No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24410.1274100782885606No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT23760.12401734781385498No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA23550.12292123489125778No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA23340.12182512196866055No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG22630.11811921637321288No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG22040.1150396610192493No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA21980.11472648589850726No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT21900.11430891907085118No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA21850.11404793980356613No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG21560.11253426005331285No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC21310.11122936371688762No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT20800.10856737519058011No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20610.10757565397489692No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT20390.10642734519884271No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA20230.10559221154353056No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT19970.1042351193536483No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT19840.10355657325870718No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19510.10183411009462585No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC19480.10167752253425484No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19220.10032043034437259No Hit
GATCTATACTAAATACTGATCAGAGAAACATGATTCTTGAAGAACAATGC19210.10026823449091557No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA19160.10000725522363052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT253.7056452E-8145.046775
TACGGTC100.007091721144.99756145
CTCTCCG100.007091721144.99756145
AGCACGC7300.0125.200241
GCACGCA7350.0124.3355562
ACAGCGT301.09946996E-7120.88184
CACGCAC7700.0118.683943
CGGTGCA900.096.705432
CACAGCG406.105038E-790.661343
ACGCACT10500.089.79794
CGCACTT10450.089.526485
ATGGTGA259.369242E-487.043971
ATATATG259.37313E-487.034883
GAAACAC7400.084.2905354
AAACACG7400.082.3238455
ACAACGC3400.074.63118
AACACGT3150.073.672636
GGTGACC300.001932936872.5290763
GGAGCCT702.8048817E-972.529074
CAACGCA3500.072.498799
Produced by FastQC (version 0.11.5)