FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9313_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9313_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723572
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG62280.36134260709735366No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC57860.3356981895737457No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT45900.26630741274515946No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG39380.2284789959456292No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC35960.2086364828391271No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG33530.19453785510555985No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT32710.18978029348353304No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG29850.17318684685061025No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA29290.16993778037703097No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA29290.16993778037703097No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT28390.1647160664016357No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA27320.1585080286753324No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG25970.15067545771223947No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC25740.14934101969630512No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC25490.14789054359202863No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG25440.14760044837117337No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25390.14731035315031807No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC24480.1420306201307517No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC23550.13663484902284326No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG23420.1358806014486195No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22200.12880227805975034No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA21730.12607538298371057No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA21710.12595934489536845No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT21280.12346452599601293No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA21180.12288433555430235No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC20450.11864894532981506No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA19950.11574799312126212No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT19880.11534185981206473No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT19830.11505176459120942No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT19140.11104845054340637No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG19120.11093241245506424No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC18540.10756730789314285No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA18470.10716117458394543No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA18160.1053625842146426No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA17960.10420220333122145No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA17540.10176540347603696No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT17420.10106917494598427No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT17370.10077907972512896No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC17260.10014087023924732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG100.007087788145.024053
TTACTCG100.007087788145.024054
GCCTACG100.007087788145.024052
AGCGAAA3250.0116.0495451
GAGCGAA600.0108.796451
CGAAAGC3750.0100.550013
ATGCGTA301.5998547E-596.665889
GATAATT1800.080.5689242
TAAACTA2200.072.5099265
GATGCGT406.6648245E-572.4994058
AAACTAT2250.070.894486
AATTCTA2150.067.451095
CAGTACG3800.066.7757649
TTTAAAC2550.062.55943
GTACTGA2600.061.350996
TACTGAT2850.055.9644557
CAGGGTG2600.055.7768675
ATAGTTG651.0940315E-555.7687768
GTATTAA5600.055.693421
CACTGTG17750.053.5066077
Produced by FastQC (version 0.11.5)