FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9108_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9108_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395454
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC734880.39949000443261684No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG627210.34095923916854676No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT515790.2803899267721254No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT463190.2517959056623446No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA440750.23959723962235455No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG409160.2224245185794273No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG405940.22067408610844833No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC397250.21595009288707961No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC374090.20336002579767806No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG373860.20323499490689387No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG354500.19271065557827494No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC341960.18589375396769223No Hit
GTGTTATCATTCCACTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT338070.18377910107573317No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG310200.16862861878809843No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC304740.16566049416339493No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA300480.1633447046210439No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA293130.15934915224163534No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC290100.1577020061586955No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT276670.15040128936203478No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA273460.14865629301674207No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG263650.1433234537185111No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG263110.14302990293145254No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG251980.1369794950426339No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC248960.13533778508538036No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC245970.13371238350518558No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA245520.13346775784930343No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA227090.12344897820950762No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAGCTCGTCCTTTATGA224510.12204645778244995No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC222210.12079614887460784No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT216580.11773561011323774No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT216090.11746923995461052No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA213330.11596886926519999No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT210970.11468594360324023No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC210570.11446849857578943No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT209060.11364764359716265No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA209010.1136204629687313No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA207190.11263108809383014No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC204540.11119051478696858No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT203650.11070669960089052No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA203460.1106034132128514No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG200540.10901606451246051No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA200080.1087660027308921No Hit
TTCTTAAACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT193700.10529775454305176No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC193660.10527601004030669No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT193120.10498245925324812No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG189300.10290585924109294No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA185830.1010195236279572No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA184880.10050309168776156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA61800.086.7314451
GAGCGAA24700.082.514441
CGAAAGC67900.079.1410453
TCACGCC903.6379788E-1272.499146145
TAACCGT851.6916601E-1068.251185
TTTAAAC33800.066.7262953
TAATAGT9150.066.575054
GCAGGGG25350.065.778319
CAGTACG59100.065.752199
TATAATA9200.065.424972
GTAACCG1009.094947E-1265.267334
AATAGTG9800.061.417365
AAACTAT37750.059.7397886
CTCGTGA502.0085304E-458.01544
GTATTAA85350.057.786451
TATATTC76350.056.419693
AGGCTCC2700.056.388226145
TATTATA8000.055.295932
ATTAATT89250.055.171513
GCAGGGT102450.053.9939969
Produced by FastQC (version 0.11.5)