FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9105_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9105_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748070
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG74740.4275572488515906No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC54180.3099418215517685No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT48870.27956546362559853No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG41450.23711865085494285No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC41120.23523085459964416No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG39400.22539143169323883No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT36160.20685670482303342No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG33420.19118227530934115No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT33400.19106786341508064No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG30790.17613711121408182No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC29440.16841430835149623No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA27110.15508532267014477No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC26860.1536551739918882No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC25780.14747693170181972No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA24340.13923927531506175No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA24310.13906765747367095No Hit
GTCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC23220.13283220923647224No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC23210.13277500328934197No Hit
CTCATAGACAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC22800.13042955945700116No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA22640.12951426430291693No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA22610.12934264646152616No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT22210.1270544085763156No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT22160.12676837884066428No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA21850.1249949944796261No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA21830.12488058258536558No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT21770.124537346902584No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG21590.12350763985423925No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG21450.12270675659441556No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA21060.12047572465633527No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC20740.11864513434816683No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA20580.11772983919408261No Hit
GATAATGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT20510.11732939756417078No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT20400.11670013214573786No Hit
CTAATAGATGCTCTATTGGGAGACCCGCAGTGTGATGGCTTTCAAAATAA20030.11458351210191813No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA19950.11412586452487601No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT19810.1133249812650523No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA19620.1122380682695773No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19340.11063630174992993No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA19220.10994983038436676No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT19070.10909174117741281No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC19050.10897732928315228No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG18950.10840526981184964No Hit
CTCATTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC18880.10800482818193778No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC18320.10480129514264304No Hit
AAACAAGGTAGTTTTTTACTCCAATTCTATGCTGACAAAATGACTGTCGT17720.10136893831482721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4000.097.934881
AGACGCC750.096.66495145
CGAAAGC4600.085.1437153
CGTAGTT554.3968612E-879.08951145
CTAATAC608.0122845E-872.529833
TATATTC3300.063.738343
ATATTCA3650.059.6135644
TTATATT3550.059.249722
GTATTAA4800.058.9422951
CCAACCC753.7633072E-757.998978
CACTGTG15300.057.3355837
CAGGGTG2750.055.3626569
GTCCAAC805.865386E-754.389596
ATTAATT5250.053.8793033
GAACTGG553.215407E-452.741426
GCAGGGT3950.049.556088
GCCCGGA2050.049.51132145
AACACTG22250.049.2196775
AATTCTA15650.048.6594055
ACAAGGT7900.048.6383748
Produced by FastQC (version 0.11.5)