FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9060_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9060_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641998
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG76810.467783761003363No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT67340.4101101219368112No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC60300.3672355264744537No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT59420.361876202041659No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT55820.339951692998408No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC55690.33915997461629066No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC55210.3362367067438572No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG53020.3228992970758795No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA49110.29908684419834863No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA48190.2934839141095178No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA47230.28763737836465086No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG45810.27898937757536857No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT45520.2772232365691067No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA41640.25359348793360287No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT40550.24695523380661852No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG39960.24336205038008574No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC39550.24086509240571546No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA39450.24025607826562517No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA39130.23830723301733622No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT38520.23459224676278534No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT37750.22990283788409No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT37300.22716227425368363No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT35760.21778345649629294No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC35070.2135812589296698No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT34410.2095617656050738No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC33720.2053595680384507No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT33580.2045069482423243No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT33450.20371522986020688No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA32990.20091376481579148No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA32620.19866041249745736No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA32290.19665066583515936No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31850.19397100361876202No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT31310.19068232726227438No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30810.18763725656182284No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA30400.1851402985874526No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG29380.1789283543585315No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA28980.17649229779817027No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA28510.17362993133974586No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG28200.1717419875054659No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA27850.16961043801514983No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG27670.1685142125629873No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT27660.16845331114897827No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA27550.16778339559487893No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG27330.16644356448668024No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26780.16309398671618358No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA26560.16175415560798492No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC26440.16102333863987653No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT26140.15919629621960563No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT25340.15432418309888318No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG25280.153958774614829No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA25150.1531670562327116No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG24460.14896485866608852No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT23680.14421454837338413No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG23670.1441536469593751No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA23490.14305742150721254No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC23290.14183939322703196No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT23080.1405604635328423No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA23000.14007325222077008No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22740.13848981545653527No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT22550.13733268859036368No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG22540.13727178717635466No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA22340.13605375889617405No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT22250.13550564617009278No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA22150.13489663203000246No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG21900.13337409667977673No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG21340.12996361749527102No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20150.12271634922819638No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT19760.1203411940818442No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT19660.11973217994175388No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA19600.1193667714576997No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC19580.11924496862968165No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT19200.11693071489733849No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG19180.11680891206932043No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT18960.11546908096112175No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG18590.11321572864278764No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA18470.11248491167467926No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA18200.11084057349643543No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG18000.10962254521625482No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA17830.10858722117810132No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT17760.10816091128003812No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT17590.10712558724188459No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17440.10621206603174911No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG17400.105968460375713No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA17390.10590755896170397No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC17390.10590755896170397No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA17310.10542034764963173No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC17150.10444592502548723No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG16910.1029842910892705No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC16650.1014008543250357No Hit
GCGTAATGTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAAC16480.1003655302868822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG100.007083841145.050874
AGCACGC6400.0118.997921
GCACGCA6600.0115.381372
CACGCAC6700.0113.659273
GCGGGAG259.3883777E-486.9987267
GGTGCAT1100.085.7118763
GCGGTGC3100.081.891041
GAGCAGA1750.078.74911
ACAACGC4100.076.035468
ACGCACT11150.073.5011144
CGCACTT11000.073.1825265
CAACGCA4350.071.665629
TAACAAG17450.069.821795
AAGCACG1150.069.3784941
CGGTGCC2400.063.459762
AGCGGTG1550.060.8333441
CACTTTC16550.059.5761647
CGGTGCA1600.058.9269142
ATCTACA502.0107723E-457.999158
GCGGAGC4650.057.7136881
Produced by FastQC (version 0.11.5)