FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_874_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_874_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG69620.4911297536309176No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT44260.31222928606297634No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC42530.300025113788034No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT29900.2109276017461137No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC29400.20740038432561012No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG27810.1961838329284088No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA27620.19484349030861742No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT26490.18687197893827934No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC23200.16366288831136583No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT22440.1583015178322004No Hit
GAATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTTATG22260.1570317195608191No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT22110.15597355433466803No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG22010.15526811085056733No Hit
GGTCTATTCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT21610.15244633691416445No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA21040.1484253090547904No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC20500.14461591424064654No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT20390.14383992640813575No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC19820.13981889854876167No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG19820.13981889854876167No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA19470.13734984635440917No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA19380.13671494721871852No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG19180.1353040602505171No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC19000.1340342619791358No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18770.13241174196570415No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18660.13163575413319337No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT18130.1278969036674596No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG17920.12641547235084807No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG17710.12493404103423658No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC17380.12260607753670422No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT17350.122394444491474No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC16970.11971375925189129No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG16930.11943158185825099No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC16630.11731525140594885No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC15930.11237714701724386No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA15810.11153061483632301No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT15710.11082517135222228No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA15670.110542993958582No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC15620.11019027221653163No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG15600.11004918351971152No Hit
CAATATGGGTTAGCCACTCAATTCAAATTGGAAATCAAAGCCAGATTGAA15460.1090615626419705No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC15460.1090615626419705No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC15430.10884992959674028No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC15330.10814448611263956No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG15260.10765067567376908No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT14950.10546380087305685No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA14920.10525216782782665No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT14850.10475835738895614No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA14840.10468781304054607No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC14560.10271257128506407No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTATCACGATTCAAATG14510.10235984954301372No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC14510.10235984954301372No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC14410.10165440605891299No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG14390.10151331736209285No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT14380.10144277301368278No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA14220.10031406343912165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA4700.092.594531
ACATAAG950.091.603682
TCACGCC406.119244E-790.62389145
GATTTAG353.4301676E-582.894151
GAGTTTA451.2332421E-680.554577
CGAAAGC4350.080.0216143
GCGAAAG4650.074.858922
TAAACTT300.001933836672.519584
GTACTGA502.3040066E-672.5144656
AGCGGAA300.00193600372.499129
GTAGAAA8350.066.0174941
GATAGCG350.003563992762.1552546
TAGCGGA350.003566982562.1421058
GGACAAT1251.8189894E-1258.011576
GGATCGC400.00604663254.385856
GGGTGGG400.006051697354.3743367
TCGCAGG400.006051697354.3743369
TTCTACT953.133937E-853.4354824
CCTTATA2200.052.7415162
GAAGTGC701.6964697E-551.785088145
Produced by FastQC (version 0.11.5)