FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_822_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_822_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762253
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT42070.23872849131197393No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG41620.23617494196349784No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC39790.22579050794636185No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT36900.20939104657503776No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC36870.209220809951806No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG34140.19372927723771785No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC31860.18079129387210574No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA31590.17925916426302013No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA29340.16649141752063978No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG28830.16359739492570022No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28720.16297319397385052No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT28320.16070337233076068No Hit
GTCCTACATTGTGGAAACATCTAATTCAAACAATGGAACGTGTTACCCAG27820.15786609527689838No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA27600.15661769337319895No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTCCCCCTGC26540.1506026660190109No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA26020.1476518978829941No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT25750.14611976827390843No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC25540.1449281119112863No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA24890.14123965174126532No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT23820.13516787884599998No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT23460.13312503936721912No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC23320.13233060179213768No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC23230.13181989192244248No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT22800.12937983365612088No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC22620.12835841391673045No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC22600.12824492283457595No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC22270.12637231997902684No Hit
CAATATGGGTTAGCCACTCAATTCAAATTGGAAATCAAAGCCAGATTGAA22180.12586161010933164No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC22130.1255778824039454No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21610.1226271142679286No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC21520.12211640439823339No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG21320.12098149357668848No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC21200.12030054708376152No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG21180.12018705600160702No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT20720.1175767611120537No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG20640.11712279678343575No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20260.11496646622250041No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC20000.113491082154492No Hit
GTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC19870.11275339012048781No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA19760.1121291891686381No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG19570.11105102388817042No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT19260.10929191211477579No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG18940.10747605480030394No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC18760.1064546350609135No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAA18740.106341143978759No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC18710.10617090735552727No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT18570.10537646978044583No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA18540.1052062331572141No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG18380.10429830449997816No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGAT18340.10407132233566917No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT18110.10276617489089251No Hit
GGGTTAAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA18070.10253919272658353No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18010.10219871948012006No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG17920.10168800961042485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC3600.0104.753523
ACGGGGG352.759116E-7103.593576
GTACTGA3850.097.9436
AGTAGAA10550.097.628721
GCGAAAG6350.093.650032
CGAAAGC6650.090.515773
GCAGGTA5000.081.2242662
CCGCACC554.3968612E-879.08987145
CTAGTTG300.001933694272.521673
AGGTACT5800.067.520174
GTAGAAA15950.064.564752
GAAAGCA9950.059.7666054
AGCGAAA10500.056.645621
GGTACTG6750.055.866965
GCAGGGT4750.054.946648
GGGCCAC400.006045176654.389715
TTTTAAA8750.053.873242
AGGTCGT5250.051.106166
CAATTCG9150.050.7097059
TAGAAAC21250.050.1679273
Produced by FastQC (version 0.11.5)