FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_753_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_753_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956657
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA108420.5541083593087598No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC88940.4545507976104141No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA78830.40288103637990713No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC72420.37012107896274105No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG62230.31804245710924295No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA60900.3112451492520151No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT47350.24199438123288855No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT46410.23719026891274247No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA42660.21802492721003222No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT40950.20928553139359635No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT40130.20509471000793703No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG37190.19006908211301213No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT36020.18408949550176654No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC35780.18286291363279308No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT35210.1799497816939811No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA33180.16957494338558063No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT33150.16942162065195893No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC32670.16696845691401202No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG32100.16405532497520003No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA32030.16369757193008277No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG29860.15260722753144776No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG29760.15209615175270885No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT29720.15189172144121327No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29540.15097178503948316No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG28480.1455543817848504No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG28060.14340786351414683No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC28010.14315232562477737No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT27480.140443623997461No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA27020.13809267541526185No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26740.1366616632347928No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG26170.13374853129598085No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA26040.13308413278362025No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG25650.13109093724653834No Hit
AGTAGAAACAACGCACTTGAACTTTATGTTAAATCCAGCAATCATGCTAA25480.13022210842268217No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT24260.12398698392206708No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT23760.12143160502837239No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT23180.1184673655116865No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22970.11739410637633474No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22920.11713856848696526No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22820.11662749270822632No Hit
GATAAGAACCAGCCTTGATGGACTGGTTCCTGTTGTGCTTACCGTTTTAT22800.11652527755247853No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA22570.11534980326137897No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT22450.11473651232689225No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA22380.11437875928177497No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT22370.11432765170390109No Hit
GTATGCTGCCATTGCAAGAATCGCGTAAAGAATAGCCATCACTTCACCAG22250.11371436076941437No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT22040.11264110163406257No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT21870.1117722728102064No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21770.11126119703146745No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC21630.11054569094123294No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC21620.11049458336335903No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA21230.10850138782627716No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC21190.10829695751478159No Hit
GTATTATTGCTATTGGTTTAACACCAGCATTGCTTGTTGTTTGCCGCGGT20970.10717259080155592No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG20940.10701926806793423No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC20880.10671262260069088No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG20840.10650819228919531No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC20580.10517939526447405No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC20260.10354395277250943No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA20240.10344173761676165No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA20230.10339063003888777No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG20200.10323730730526608No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA19910.10175518754692314No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA19630.10032417536645412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAG259.3742006E-487.0324551
GGTGCAT1000.087.0191043
GTAACAA32850.086.0919654
CGGTGCA1100.085.700642
GAGCCCC300.001936100372.499245145
TAACAAG39400.071.595675
AACAACG12750.070.509077
TAGTAAC42500.068.4209062
ACAACGC13700.065.619768
CAACGCA14150.063.532919
AGTAACA45950.063.2837563
AAACACG13450.060.924165
AACACGT5300.060.2003636
GAAACAC13800.059.904464
AGCACGC13050.059.466621
GCACGCA13400.057.9045072
CACGCAC13900.056.865013
CAAGAGG23950.056.6069268
ACGTGCA3700.054.8769152
CGGAGAC400.00604649254.3869443
Produced by FastQC (version 0.11.5)