FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_709_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_709_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759269
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA43810.24902388435196665No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC41230.23435870239286888No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT34110.1938873475290021No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT32380.18405371776573112No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA31340.1781421715496607No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30100.1710937895228075No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT29950.1702411626647204No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT29930.17012747908364212No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC27400.15574650607724005No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA27040.15370020161783104No Hit
ATATTACTCTTAGTGCTTTCCCAAAGGATCCTTTTACTAGTTTCCCTTCT25300.1438097300640209No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25260.14358236290186435No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25030.14227500171946417No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24310.13818239280064618No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG24140.1372160823614808No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA23430.13318031523320198No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23250.13215716300349747No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22360.12709824364551411No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA20890.11874250043626075No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC20870.11862881685518246No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20810.11828776611194763No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT19420.11038675722700735No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC19350.10998886469323339No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA19230.10930676320676372No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA18950.10771519307166784No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA18660.10606678114603281No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA18410.10464573638255435No Hit
TTCTAATACTTTACTTCCCTCTTTTTCAAAACCCAACCATTCATTGAACC18250.10373626773392812No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT18170.10328153340961502No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC18040.1025425901326062No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17750.10089417820697119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGT100.0070861722145.03525
GAAACAC5200.099.0200354
AAACACG5450.094.4724665
AGCACGC3400.091.73691
AACACGA3700.090.151886
ACACGAG3800.087.7627
CGTGCGC502.2650056E-887.026063
ACGTGCG502.2650056E-887.026062
GTGCGCA502.2650056E-887.026064
GCACGCA3600.086.623172
AACACGT2300.081.9717566
CACGCAC3850.080.998283
ACGTGCC1650.079.089879
TAGAAAC7200.078.565192
ACACGTG2450.076.937777
CACGAGC4400.075.7944648
ACGAGCC3800.074.406919
AACAACG2300.069.346927
ACAACGC2350.067.871458
CAACGCA2350.067.871459
Produced by FastQC (version 0.11.5)