FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_709_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_709_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768890
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA44010.2488000949748147No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC41360.23381894860618804No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT34300.19390691337505442No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT32540.18395717088117408No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA31480.1779647123337234No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30290.17123732962479296No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30120.17027627495208858No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT30110.17021974232428247No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC27590.1559735201171356No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA27130.15337301923805324No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25430.14376247251100974No Hit
ATATTACTCTTAGTGCTTTCCCAAAGGATCCTTTTACTAGTTTCCCTTCT25420.14370593988320357No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25120.14200996104901945No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24430.1381092097303959No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG24210.13686549191866085No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA23530.13302127322784343No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23370.13211675118294525No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22440.12685921679697437No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT21000.11871851839289045No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA20990.11866198576508433No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC20970.11854892050947204No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT19480.11012555896635742No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC19430.10984289582732674No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA19290.1090514390380408No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA19050.1076946559706935No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA18780.10616827501992775No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA18480.10447229618574361No Hit
TTCTAATACTTTACTTCCCTCTTTTTCAAAACCCAACCATTCATTGAACC18300.10345470888523312No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT18210.10294591523497788No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC18140.1025501868403349No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17830.10079767537834461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG100.007083916145.050666
TAGACGC100.0070917048144.997367
CCTCACC100.0070917048144.99736145
AGCTAGG100.0070917048144.997367
ACGTGCG256.46684E-6115.997919
CCACGAA203.866833E-4108.748024145
AACACGT2200.0102.1947866
ACAACGC2300.0100.867748
AAACACG5650.097.5562065
CAACGCA2400.096.664929
ACGTGCC1650.096.664919
ACACGTG2350.095.636567
GAAACAC5800.095.0385744
GCACGCA3550.089.8956452
AGCACGC3600.088.664641
AACACGA3900.087.402326
CACGTGC2600.086.4407358
AACAACG2700.085.924377
ACACGAG3900.085.511277
CGGTGCG850.085.323935
Produced by FastQC (version 0.11.5)