FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_703_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_703_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374600
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT101970.7418158009602793No Hit
GTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA62300.4532227557107522No Hit
GTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACACAC56130.40833697075512876No Hit
CACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTG48480.3526844172850284No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGG43320.31514622435617634No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGAC41680.3032154808671614No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTA37140.27018769096464423No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC35070.25512876473155827No Hit
CACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTGTCCT34270.24930888985886807No Hit
GTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACA33090.24072457442164993No Hit
GTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCC32450.23606867452349772No Hit
GTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAA31460.22886657936854354No Hit
GTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACAC30770.22384693729084826No Hit
GTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGT30230.21991852175178234No Hit
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG29640.21562636403317328No Hit
GTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGC28270.20565982831369126No Hit
CTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACA27420.1994762112614579No Hit
CACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTCCT25580.18609049905427033No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC22920.16673941510257528No Hit
CACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTG22790.16579368543576314No Hit
CACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTG21900.15931907463989523No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG21720.15800960279353995No Hit
CACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGT21470.15619089189582425No Hit
CACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCT20960.15248072166448423No Hit
CACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACG20650.15022552015131677No Hit
CACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACA20540.14942528735632185No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT20270.1474610795867889No Hit
CACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGA19880.14462389058635242No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT19740.1436054124836316No Hit
CACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGA19720.14345991561181434No Hit
CACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACT19270.14018623599592608No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC19150.13931325476502254No Hit
CACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGT19090.13887676414957079No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG19000.13822202822639315No Hit
CACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAG18650.13567583296959115No Hit
TCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCC18270.1329113924050633No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC18230.13262039866142877No Hit
GTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACAC18200.1324021533537029No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17760.12920122217372326No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA17510.12738251127600758No Hit
GTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTG17380.1264367816091954No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC17370.12636403317328676No Hit
CACACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAG17060.12410883166011932No Hit
AACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACC16930.12316310199330714No Hit
GTTCTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA16660.12119889422377418No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA16550.12039866142877927No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT16390.11923468645424125No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT16340.1188709442746981No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCAC16140.11741597555652553No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA16100.11712498181289102No Hit
ACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACCA15930.11588825840244436No Hit
CACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTC15910.1157427615306271No Hit
CACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTC15890.11559726465880983No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA15850.11530627091517533No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG15620.11363305688927688No Hit
CACACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGC15240.11086861632474902No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA15140.11014113196566273No Hit
ACGCTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACAC14800.1076676851447694No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG14330.10424850865706388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAA750.087.036931
CGAAAGC9450.073.6713263
CAGTACG3250.073.614489
GGGTGAC4550.065.357391
GCGAAAG10750.064.762242
AAACGTC1051.6370905E-1162.155666
TATATTC3450.052.550883
GTACTGA1800.052.3718956
ATATTCA3200.052.1239054
AAGGTAC858.9280366E-751.175849
TAGAAAC10500.049.037482
TCTATGC7100.048.006343
GCCCGGA1255.1986717E-946.39943145
GCATTAT1751.8189894E-1245.5907671
ATATAAG1602.0008883E-1145.331731
GTACGAG657.3222275E-444.634321
AGCGAAA15650.044.4917141
TGACCCC2450.044.38721145
TAAACTA4250.042.65745
GAGGCGG855.3349097E-542.6573945
Produced by FastQC (version 0.11.5)