FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_703_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_703_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381741
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT102440.7413835154345134No Hit
GTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA62520.45247264139950977No Hit
GTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACACAC56430.40839781116721585No Hit
CACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTG48710.35252626939491555No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGG43530.31503733333526324No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGAC41810.30258926962433624No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTA37310.2700216610783063No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC35190.25467869882995436No Hit
CACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTGTCCT34450.24932313653571836No Hit
GTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACA33200.24027657860626558No Hit
GTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCC32640.23622372065387073No Hit
GTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAA31580.22855223952969478No Hit
GTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACAC30890.22355853955263685No Hit
GTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGT30360.21972279899054886No Hit
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG29770.21545282364784715No Hit
GTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGC28410.20561016862060255No Hit
CTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACA27610.1998203715457528No Hit
CACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTCCT25650.18563536871237085No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC23050.16681852821910909No Hit
CACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTG22870.16551582387726788No Hit
CACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTG21960.15892992970462624No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG21820.15791671521652756No Hit
CACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGT21520.15574554131345889No Hit
CACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCT21070.1524887804588559No Hit
CACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACG20770.15031760655578721No Hit
CACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACA20640.14937676453112414No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT20340.1472055906280555No Hit
CACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGA19950.14438306455406621No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT19830.14351459499283875No Hit
CACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGA19810.1433698500659675No Hit
CACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACT19360.1401130892113645No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC19260.1393893645770083No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG19160.13866563994265205No Hit
CACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGT19130.1384485225523452No Hit
CACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAG18710.13540887908804908No Hit
TCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCC18360.1328758428678023No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC18290.13236923562375294No Hit
GTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACAC18260.13215211823344605No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17820.1289677298422787No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA17570.12715841825638813No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC17440.12621757623172505No Hit
GTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTG17420.1260728313048538No Hit
CACACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAG17100.1237569124749139No Hit
AACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACC16970.12281607045025081No Hit
GTTCTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA16690.12078964147405338No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA16620.12028303423000403No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT16480.11926981974190531No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT16400.11869084003442035No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCAC16190.11717101830227226No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA16170.11702627337540103No Hit
ACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACCA15980.11565119657012421No Hit
CACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTC15960.11550645164325297No Hit
CACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTC15950.11543407917981735No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA15940.11536170671638173No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG15670.11340765020361993No Hit
CACACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGC15280.11058512412963066No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA15190.10993377195871006No Hit
ACGCTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACAC14880.10769022559220577No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG14380.10407160242042465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC9650.075.915023
TCTAGAA702.8030627E-972.532682
ATATTCA3600.066.488294
CCCATAA554.0472223E-665.950741
GCGAAAG11400.064.2614062
ATCGAGT350.003561962662.1641126
TATATTC3900.061.3738063
CAGTACG3650.055.615339
TAGAAAC10000.054.399512
AAACGCC400.006051849654.373917145
TCGAGTT400.006051849654.3739177
GATTACC553.2197498E-452.726227
CTAATCG450.00962276348.3551183
GTACTGA1501.0913936E-1148.3498656
GTGGCCG752.5513948E-548.3323679
GGGTGAC4100.047.7740671
CGTGGGC1551.4551915E-1146.7952772
GGTAGTT2700.045.6472367
GTACGAG657.314765E-444.6435741
AGTAGAA7500.044.4947621
Produced by FastQC (version 0.11.5)