FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_581_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_581_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195827
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC7730.39473617019103596No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT7150.36511819105639165No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG6920.3533731303650671No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG5730.2926052076577796No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC4920.25124216783181075No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4660.23796514270248736No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT4610.235411868639156No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC4340.2216241886971664No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4320.22060287907183382No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA4220.215496330945171No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG4200.21447502131983842No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC4050.2068151991298442No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT4040.2063045443171779No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTATGC3950.20170865100318136No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG3630.18536769699786035No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT3620.18485704218519405No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3440.175665255557201No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT3350.17106936224320446No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA3290.16800543336720677No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT3280.1674947785545405No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC3280.1674947785545405No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG3020.15421775342521715No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA3010.15370709861255086No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC2950.15064316973655317No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC2850.14553662160989037No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG2840.1450259667972241No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG2840.1450259667972241No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT2780.14196203792122639No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC2760.14094072829589382No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA2740.13991941867056126No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC2720.1388981090452287No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT2720.1388981090452287No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT2710.1383874542325624No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC2710.1383874542325624No Hit
AAACTATTCTCTGTCGCTTTCAATCTATGCCGTATTTCTTCAATTAACCA2660.13583418016923102No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC2650.13532352535656472No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT2620.13379156091856587No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG2610.1332809061058996No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2600.1327702512932333No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG2540.12970632241723562No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT2540.12970632241723562No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT2490.1271530483539042No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT2490.1271530483539042No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2480.12664239354123794No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC2480.12664239354123794No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG2470.12613173872857164No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG2450.12511042910323908No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC2440.1245997742905728No Hit
CTTTCAATCTATGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT2410.12306780985257394No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2400.12255715503990768No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAGCTGATTGCCCC2400.12255715503990768No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG2400.12255715503990768No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA2390.12204650022724138No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT2340.11949322616390998No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC2330.11898257135124368No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC2280.11642929728791229No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC2250.11489733284991345No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG2250.11489733284991345No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG2240.11438667803724716No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG2240.11438667803724716No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA2230.11387602322458089No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT2230.11387602322458089No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG2210.11285471359924833No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTTATG2210.11285471359924833No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA2210.11285471359924833No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA2130.10876947509791807No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT2130.10876947509791807No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG2100.10723751065991921No Hit
GTAGAGACCCATTAGAACACATCCAGAAGCTGATTGCCCCCAGGGAGACT2100.10723751065991921No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA2080.10621620103458665No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG2070.10570554622192038No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC2060.10519489140925409No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTCCCCCTGC2050.1046842365965878No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT2040.10417358178392153No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG2030.10366292697125525No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2020.10315227215858895No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT2010.10264161734592268No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC2000.10213096253325639No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC1990.10162030772059012No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC1980.10110965290792383No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT1970.10059899809525756No Hit
TCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGC1970.10059899809525756No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT1960.10008834328259127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG500.0130.499542
CGAAAGC500.0130.499543
TCGTTTT301.09839675E-7120.832919
AGGTCGT301.09839675E-7120.832916
GGTCGTT301.09839675E-7120.832917
ACTCAGC451.0832082E-896.715711
CTCAGCA451.0870281E-896.666332
GCACCGT451.0870281E-896.666336
CACCGTC502.2591848E-886.999697
ACCGTCT502.2591848E-886.999698
AGTAGAA651.549779E-978.116551
CAGGGTG651.5552359E-978.0766459
GTAGAAA1400.077.717991
AAGCATC300.00193343772.499756
GCAGGGT702.7976057E-972.499748
CAATTCG554.0495106E-665.908869
TTATACT554.0495106E-665.908864
CCTTATA554.0495106E-665.908862
ATATTCA554.0495106E-665.908864
TTATATT702.324814E-762.1426355
Produced by FastQC (version 0.11.5)