FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_463_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_463_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242741
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTT6970.2871373192002999No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG6540.26942296521807196No Hit
GTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCA6010.24758899403067466No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC5790.2385258361793022No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT5540.22822679316637898No Hit
TATCCACACTGTGTGTTCACAACTTTTTGCGCAGGATCTGGCACGCAGCA5380.2216354056381081No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG4380.18043923358641514No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT4250.17508373121969506No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT4160.1713760757350427No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA4090.16849234369142418No Hit
CTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCC3920.1614889944426364No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAATC3470.14295071701937456No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG3280.1351234443295529No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC3250.13388755916800213No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA3210.1322397122859344No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3170.13059186540386666No Hit
ATCTAAGGCTGCTCAGAGGAGAATGCTGGAGGAGAGGAAGAGGAAGTGAA3090.12729617163973123No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC3070.12647224819869737No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCCCGTTGGGAAACAAACTGTG3070.12647224819869737No Hit
AGCAGGGACCGCCCACAGTGTTTCTACCAATTTTCAAAACCGTTTGATTC3070.12647224819869737No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3040.12523636303714658No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT2940.12111674583197729No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG2840.11699712862680799No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC2820.11617320518577413No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2710.1116416262600879No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT2580.10628612389336782No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC2530.10422631529078319No Hit
CTTTAAGAAAAGGAGAGCCGGCTCTCAGTGAAGAACCATGCCCACCGCGC2500.10299043012923238No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG2450.10093062152664774No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT2450.10093062152664774No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA2450.10093062152664774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCA100.007087638144.999578
CGAAAGC1700.0110.8820343
GCGAAAG2200.085.681572
ATATACC353.432072E-582.85696
TACCTTA353.432072E-582.85699
AGCTCTA451.2300861E-680.555325
GTAGAAA1300.078.108881
GGACCGC651.5570549E-978.07676
CCGCCCA651.5570549E-978.07679
TATATCT300.001933992472.4997944
ATCACAC300.001933992472.4997943
TCTAGCT406.653127E-572.499792
TTATATA502.3010125E-672.499794
ACCGCCC754.834874E-967.666478
TATACCT451.1920612E-464.444267
CTCTATG451.1920612E-464.444267
ATACCTT451.1920612E-464.444268
TCTATGT451.1920612E-464.444268
TAGGTTA1500.062.8331576
AGGTTAT1500.062.8331577
Produced by FastQC (version 0.11.5)