FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_463_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_463_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715853
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTT49060.2859219292095535No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG45190.26336754955115615No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT41380.24116285019754025No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC40560.23638388603219504No Hit
TATCCACACTGTGTGTTCACAACTTTTTGCGCAGGATCTGGCACGCAGCA39050.22758359836186434No Hit
GTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCA38970.2271173579554892No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG31790.18527228148332053No Hit
CTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCC29440.17157646954605085No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT29310.17081882888569125No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA28210.1644080232980331No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT27250.15881313842153144No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAATC24460.1425530042491985No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA23580.13742435977907197No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC23150.1349183175948056No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG22360.13031419358185112No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA22320.13008107337866356No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC20730.12081454530195768No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCCCGTTGGGAAACAAACTGTG20650.12034830489558254No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20550.11976550438761362No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20330.118483343270082No Hit
AGCAGGGACCGCCCACAGTGTTTCTACCAATTTTCAAAACCGTTTGATTC20260.11807538291450374No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG20240.11795882281290997No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC20100.11714290210175349No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT19860.11574418088262806No Hit
ATCTAAGGCTGCTCAGAGGAGAATGCTGGAGGAGAGGAAGAGGAAGTGAA19190.11183941747923626No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG17870.1041464507740465No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT17550.10228148914854594No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT17520.10210664899615526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC10200.090.289823
GTAGAAA7100.073.548311
GCGAAAG12600.073.091762
GGACCGC4100.072.507796
CCGCCCA4300.069.127279
CTATTAG1050.069.073141
TCTATAC451.1927786E-464.458882
ATCAGAT451.19415556E-464.443857
ACCGCCC4750.062.578378
CTATACC350.003563775462.1567763
GTTACGC3100.056.14163
CAATTCG2250.054.777279
GCATCGC953.135574E-853.4330142
GTTAAAT2250.051.5746151
GCTATGA1004.697722E-850.7613642
TCGCCTA1004.7008143E-850.7569275
CTATATA4750.050.37972
TCTACTA1301.3096724E-1050.199165
TACTAAT1301.3096724E-1050.191857
AGCGCTT1750.049.719626
Produced by FastQC (version 0.11.5)