FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_455_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_455_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391632
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG66240.4759879048484082No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA44360.31876243144739413No Hit
TTATAAAAGCTTGTGTTGTAGTATGGTTAAAGAAACAGCACAGAACTTAC36940.26544373799970106No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA35080.25207813559906644No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT33590.24137128206307415No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT33250.23892810743070006No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT32140.23095186083677294No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32050.23030513813996806No Hit
TGTAAGCAGAGTGCAGAAAACATTTTTGTAATGTGTGTGTTTGACTGGGT30980.222616323855732No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA29930.21507122572634146No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC29800.21413707071984547No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT29010.20846028260344687No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC28320.20350207526127598No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT25880.18596870437012084No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG25760.18510640744104764No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25530.183453671660324No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC23370.16793232693700633No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT23360.16786046885958358No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT23020.1654172942272095No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA21700.15593202800740427No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA20580.14788392333605435No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC20360.14630304563275348No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT19960.14342872253584282No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT19340.13897352173563127No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA19170.13775193441944422No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG18690.1343027467031514No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA18390.1321470043804684No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT18340.13178771399335457No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT18040.12963197167067156No Hit
GTAGTAACAAGAGCATTTTTCAGAACCAATCAAGTTCAGTAAGGACAATT17770.12769180358025686No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT17450.12539234510272831No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT17310.12438633201880957No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC17120.123021028547777No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16950.12179944123158995No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC16740.12029042160571185No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA16710.12007484737344355No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16530.11878140197983376No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA16430.11806282120560609No Hit
GAACTAATGATATTCAGCTACAATCAAGACTATTCGTTAAGTAATGAATC16370.11763167274106948No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT16220.11655380157972797No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC16050.11533221426354094No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG15830.11375133656024007No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT15760.11324833001828069No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15690.11274532347632132No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT15450.11102072961817493No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT15210.1092961357600285No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT15150.10886498729549192No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGG15130.10872127114064639No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA15000.10778711613415042No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA14870.10685296112765442No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG14820.1064936707405406No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA14710.10570323188889016No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC14620.10505650919208527No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA14600.10491279303723973No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT14550.10455350265012589No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT14420.10361934764362991No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGC14180.10189475378548352No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC14060.10103245685641031No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA14050.10096059877898755No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC14000.10060130839187373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG151.2282563E-4145.054862
CGCACTG501.8189894E-12116.039725
AGCACGC2750.0110.801021
GCGGTGC501.9463187E-10101.5676041
GCACGCA3100.0100.6025542
CACGCAC3700.084.288633
CGGTGCA353.431308E-582.888482
AGCGTTT451.2314231E-680.574456
TAGTAAC13400.076.8574142
GTAACAA13000.076.432754
GCACTGC851.6916601E-1068.251296
ACGCACT5850.066.9483954
GTAGTAA16450.066.153451
GCGTTTT554.062851E-665.907977
GGTGCAT451.1917892E-464.468833
CGCACTT5100.063.9924855
TTTACAC4300.060.7206344
CAACGCA4050.057.2829749
ACAGTCT4600.056.7381639
ACAACGC4100.056.58448
Produced by FastQC (version 0.11.5)