FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_440_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_440_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159717
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT94670.4383444682798719No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT58000.26855370402696277No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT55350.2562835778946964No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC55090.2550797164628514No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT52160.24151312417321344No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG51950.24054077455518477No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG49680.23003013820792262No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA46420.21493556794709678No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA43090.19951688114692806No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA42990.19905385751929536No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCA41830.19368278343875608No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT41640.19280303854625397No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT39520.18298693764044086No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA39040.18076442422780392No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG35260.16326213110328808No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT34020.15752063812064265No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC33080.1531682160208953No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31900.14770453721482954No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA31380.14529681435113953No Hit
CAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCAAACAAGC31290.1448800930862701No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT31180.14437076709587413No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT31040.14372253401718835No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30220.13992574027060029No Hit
CTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC29950.138675576475992No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA29250.13543441108256313No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT28850.13358231657203237No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA28730.13302668821887312No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT26970.1248774723725377No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26080.12075656208660672No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25770.11932118884094536No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT24480.11334818404448362No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA24360.11279255569132438No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24200.11205171788711205No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC23790.11015332101381801No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22870.10589350363959722No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA22510.10422661858011951No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT22480.10408771149182973No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG22360.10353208313867049No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT22340.10343947841314394No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC22090.10228191934406221No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT22070.10218931461853567No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG22030.10200410516748258No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG21630.10015201065695181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACG100.007081325145.069151
TATACGG100.007088683145.018775
ATACGGC100.007089174145.015416
ACTAGCC100.007091628144.99863145
GACGACG100.007091628144.998637
ACGACGT100.007091628144.998638
CGGTGCA700.0134.669652
AGCACGC12600.0128.37471
GCACGCA12750.0127.397892
CACGCAC12800.0126.9002463
GGTGCAT750.0125.6916663
GAAACAC5850.0117.758474
AAACACG6500.0105.9752665
ACGCACT15950.0105.475534
CGCACTT15950.0105.0135965
GAGCAGA1150.094.610311
GCGGTGC1800.092.683081
TAGAAAC9650.090.924832
AACACGT2450.088.784956
GTAACGG259.370464E-487.04151
Produced by FastQC (version 0.11.5)