FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_420_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_420_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272095
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT14140.5196714382844227No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT7550.27747661662287065No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA7290.26792113048751354No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT6770.2488101582167993No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA6650.24439993384663444No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT6030.2216137746007828No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT4970.1826567926643268No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT4640.1705286756463735No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA4620.16979363825134602No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA4420.1624432643010713No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC4420.1624432643010713No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG4280.15729800253587903No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG4250.1561954464433378No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA4120.15141770337565924No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG4120.15141770337565924No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT4000.1470074790054944No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC3990.14663996030798068No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3930.14443484812289825No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA3890.14296477333284333No Hit
CTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC3730.13708447417262354No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT3710.13634943677759606No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT3660.13451184329002738No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA3580.1315716937099175No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA3490.12826402543229387No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA3370.12385380106212902No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3360.1234862823646153No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT3270.12017861408699167No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT3100.11393079622925815No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT3090.11356327753174443No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2980.10952057185909334No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA2900.10658042227898344No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC2820.10364027269887356No Hit
CAAGAGCATTTTTCAATAACGTTTTTGTAATGATGACAAGCAAACAAGCA2760.10143516051379114No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC2760.10143516051379114No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA2740.10070012311876367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG100.0070843124145.025545
CGGTGCC100.0070843124145.025542
CGGTGCA100.0070843124145.025542
AGCACGC1950.0137.613651
CACGCAC1950.0137.588353
GCACGCA1950.0137.588352
ACACGTG351.897206E-9124.2847757
AACACGT351.897206E-9124.2847756
GAAACAC1050.0117.401644
AAACACG1050.0117.401645
GCGGTGC256.4448013E-6116.041771
ACGCACT2650.0101.2442554
CGCACTT2650.0101.2442555
ACAACGC700.093.2135858
CAACGCA700.093.2135859
GTCATAA651.4551915E-1189.26291
ACGTGCA651.6370905E-1189.246492
GCACAGC259.372787E-487.0153352
TAGAAAC1750.082.871742
CACTTTC3900.081.794257
Produced by FastQC (version 0.11.5)