FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_416_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_416_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401382
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC64890.4630429104983509No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT44090.31461799851860517No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT36740.26216977241037775No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG35360.25232235036556766No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT34030.2428317189745551No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC32240.23005861356860585No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG31150.2222805773158211No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC29550.21086327639430222No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC28360.2023716588339225No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT27170.19388004127354283No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG26830.19145386482772006No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC26150.18660151193607455No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA25550.18232002409050493No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA25330.1807501452137961No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG24990.17832396876797332No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT23500.16769160728480886No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG22910.16348147756999876No Hit
GGATAGAGCAGTTAAACTATACAAAAAGCTCAAAAGAGAAATAACGTTCC22660.16169752430101142No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC22470.16034171981658105No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA22210.15848640841683426No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT21980.1568451714093659No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA21560.15384812991746719No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC21340.15227825104075834No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT21230.15149331160240392No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT21010.14992343272569505No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT20180.14400070787265715No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT19410.13850613180417617No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT19290.13764983423506225No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC19200.13700761105822679No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC19150.13665082040442933No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT19140.13657946227366985No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA19040.13586588096607494No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19000.13558044844303696No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT18950.13522365778923948No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGAT18870.13465279274316352No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT18850.13451007648164456No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA18630.1329401976049357No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG18620.13286883947417621No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT18160.12958636545923952No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC18130.12937229106696105No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA17920.1278737703210117No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT17730.12651796583658131No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG17730.12651796583658131No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC17720.12644660770582183No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA17560.12530487761366993No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC16790.11981030154518896No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG16480.11759819949164467No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC16340.11659918566101178No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCAAGTGAAC16280.11617103687645483No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT16250.11595696248417635No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC16250.11595696248417635No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT16130.1151006649150624No Hit
GCTATATACATTTACAACCGCAAATGCAGACACATTATGTATAGGTTATC16020.114315725476708No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC16010.11424436734594849No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA15910.11353078603835356No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT15890.11338806977683458No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC15820.11288856286151812No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA15770.11253177220772066No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC15670.11181819090012574No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT15480.11046238641569536No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT15350.10953473071582197No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA15330.10939201445430297No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG15290.10910658193126499No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG15280.10903522380050551No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT15280.10903522380050551No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA14940.10660904735468274No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC14850.10596682417784731No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG14830.1058241079163283No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG14810.10568139165480933No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG14690.10482509408569542No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG14590.10411151277810048No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA14550.1038260802550625No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC14470.10325521520898656No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG14390.10268435016291061No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA14340.10232755950911315No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA14330.10225620137835364No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC14320.10218484324759416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCC100.0070868097145.029793
GAGCGAA1350.0128.933791
AGTAGAA3050.099.8708341
GCGAAAG5300.079.355932
GTAGAAA6300.075.9788361
CGAAAGC5700.073.7870943
ATCTGCG451.1940759E-464.4438869
GTACTGA1900.061.058646
GTGTTTT9000.057.99959
ACAAGGG10050.057.7233053
CAAGGTA4450.057.0341954
GCAGGGT11700.057.0080579
TTAACAG6150.053.048325145
CTCAGCA2600.052.9916572
CAGGTAC2200.052.7381063
ACTCAGC2650.051.9992371
TTTAAAC1550.051.4621853
TACCTCT858.914758E-751.1869853
GGTAGTT4250.051.1760337
GCACCGT2800.049.2012756
Produced by FastQC (version 0.11.5)