FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_411_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_411_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726771
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT62490.36188932985323474No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG52710.3052518255171068No Hit
ACCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGT48660.2817976442736182No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT46910.2716631215140861No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA44520.25782225900249656No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT39100.22643419422726002No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA38740.22434937811672767No Hit
CTACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAAT36060.2088290804049871No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35260.20419615571491528No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA33090.1916293474930955No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA32420.18774927306516037No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT31920.18485369513386546No Hit
ACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGC29100.1685226356013623No Hit
ATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGCAAG27450.1589672284280892No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG26390.15282860321374403No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT26160.15149663736534838No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26030.15074378710321173No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT25530.14784820917191682No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT25230.1461108624131399No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC24860.14396813474398168No Hit
ATAATAAACAGTGAAGACGAGCATCTATTGGCACTTGAGAGAAAACTAAA24740.14327319604047092No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG23740.13748204017788115No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA23510.1361500743294855No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA22940.1328491154878093No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT22870.13244373457742806No Hit
TCTCAGAGCTGACACCATAAGCTCACAAATAGAAATTGCAGTCTTGCTTT22830.13221208834292444No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA22530.1304747415841475No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22400.12972189132201084No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22010.12746334053560085No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT21830.12642093248033467No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT20780.12034021882461543No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT20650.11958736856247874No Hit
CCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGTG20500.11871869518309029No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG20450.1184291373899608No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT20450.1184291373899608No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20040.11605476348629899No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC19370.11217468905836384No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC19080.11049525385821281No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT18900.10945284580294666No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC18090.10476200955424894No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT18020.10435662864386765No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT17980.10412498240936408No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA17860.10343004370585329No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC17660.10227181253333534No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATA17550.10163478538845047No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA17540.10157687382982457No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT17300.10018699642280302No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA17280.10007117330555124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACG100.0070811957145.06921
AATAGCG100.00708549145.03985
AGCACGC8000.0130.562291
CACGCAC8100.0128.93923
GCACGCA8200.0127.366782
CGGTGCA458.367351E-11112.82182
ACACTTA203.8592052E-4108.80191
GCGGTGC1000.0101.548451
CCGGCAC301.5966923E-596.704414
ATGGACG259.3733653E-487.0339664
ACGCACT12750.084.189714
CGCACTT12700.083.940355
TCCCGGC353.4313372E-582.889482
CCCGGCA353.4313372E-582.889483
GAAACAC5800.082.53224
GGTGCAT651.5606929E-978.10743
GTAACAA18200.078.10744
ACGTGCA1250.075.4294362
ACAACGC4600.074.074978
CAACGCA4650.073.278469
Produced by FastQC (version 0.11.5)