FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_395_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_395_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785394
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT88070.4932804747859576No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47660.2669438790541471No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT43370.24291556933651617No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT41360.23165755009818562No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA36350.2035965170713019No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC35890.2010200549570571No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT34600.19379475902797927No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG33370.18690552337467248No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCA32870.1841050210765803No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT31810.17816795620462486No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30740.17217488128670758No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT29770.16674190682840875No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA29360.16444549494397315No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA28550.1599086812210638No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25610.14344172770828176No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24660.13812077334190662No Hit
CTCTAATGGTATGCTGAACAGGTCAGGACAAGGTATTTGAGCCTTTAGTC24580.13767269297421186No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24580.13767269297421186No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA23830.13347193952707356No Hit
CAAGAGCATTTTTCAATAACGTTTTTTGTAATGATGACAAGCAAACAAGC23770.13313587925130252No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23180.12983128653955373No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA23020.12893512580416422No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT22690.1270867942874234No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22640.12680674405761416No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22340.12512644267875886No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCTATCACATGTAT22230.12451033217317857No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC21890.1226059906104759No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT21510.12047760886392583No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT21210.11879730748507052No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA20810.11655690564659678No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20490.11476458417581778No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC20290.11364438325658091No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC20010.11207610196964927No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA19790.11084388095848872No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC19660.11011575036098474No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA18670.10457075581076222No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC18220.10205030374247925No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT18190.10188227360459373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT100.007079787145.0791
GAGCACG151.2274997E-4145.0791
CCGCTTA100.00708394145.050554
CCACGTT100.00708394145.050552
CGCTTAG100.007084533145.046485
GAACGCC100.0070916563144.99774145
CACCCCC100.0070916563144.99774145
AGCACGC12550.0131.78491
GCACGCA12650.0131.29082
CACGCAC12800.0129.752243
CGGTGCA850.0127.985772
GAAACAC5450.0113.112814
GGTGCAT1050.0110.514693
CACTTTA203.8668E-4108.748317
ACGCACT16350.0105.571954
AAACACG5850.0105.375655
CGCACTT16400.0105.247155
ACGTGCA4150.099.613032
TAGAAAC8200.090.214362
GAGCAGA950.083.99311
Produced by FastQC (version 0.11.5)