FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_274_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_274_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568765
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC51860.3305785123966942No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA41580.26504925849314587No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT40210.25631627426670023No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC38850.2476470344506666No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG31470.20060365956660176No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT30120.19199816416098014No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT29300.18677112250719516No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA25610.16324943506516273No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA23950.15266786293676873No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA23910.15241288529512068No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT23310.14858822067039995No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA22680.1445723228144432No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA22460.14316994578537895No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT22110.14093889142095853No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21910.1396640032127183No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC19860.12659639907825582No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19360.12340917855765522No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA18820.11996698039540658No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT18440.11754469279975012No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA17800.11346505053338135No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA17150.10932166385660058No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG16900.10772805359630028No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA16400.10454083307569967No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG15790.10065242404056696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAT100.00708603145.035682
AGCACGC5350.0132.840651
CACGCAC5550.0128.049513
GCACGCA5600.0126.906212
CGGTGCA600.0120.863062
GTAACAA17150.0113.745184
GAAACAC4600.0105.623814
AACACGT1600.0104.23116
AAACACG4850.0100.166515
ACGCACT7200.098.704844
CGCACTT7200.098.6922455
GTTAATA259.3773915E-487.024181
ACGTGCA2550.085.315112
TAGTAAC24550.079.754852
TAGAAAC6950.078.256662
ACACGTG2150.077.557447
TAACAAG25450.076.6397255
CAACGCA1700.068.234689
CACGTGC4000.065.2681351
AGTAACA30450.064.301533
Produced by FastQC (version 0.11.5)