FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_225_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_225_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222871
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT8120.36433632011342887No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT6180.2772904505296786No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC4560.20460266252675316No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA4460.2001157620327454No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT4250.19069327099532912No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3890.17454042921690127No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT3530.15838758743847337No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA3430.15390068694446563No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC3390.15210592674686252No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT3350.15031116654925944No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC3180.14268343570944628No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3170.1422347456600455No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAACCAAG3040.13640177501783543No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2890.1296714242768238No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA2870.12877404417802224No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2800.12563321383221684No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2720.12204369343701066No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA2660.119351553140606No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2630.11800548299240368No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA2570.11531334269599904No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2500.11217251235019361No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC2450.10992906210318973No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2420.10858299195498741No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2400.10768561185618586No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAACCAAGCA2320.10409609146097966No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA2290.10275002131277734No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT2260.10140395116457503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA151.226613E-4145.062851
TAGTAGA100.0070780125145.062853
AGCACGC1100.0131.875321
CACGCAC1150.0126.141613
GCACGCA1150.0126.141612
GAAACAC750.0125.721144
GTAACAA2450.0118.418654
AAACACG800.0117.863575
ACGCACT1300.0111.586814
CGCACTT1300.0111.586815
ACGTGCA404.783942E-9108.797142
GAGAATA203.8623097E-4108.748329
ACCGTCG301.5968424E-596.665179
TACCGTC301.5968424E-596.665178
ATACCGT301.5968424E-596.665177
CATACCG301.5968424E-596.665176
TAGAAAC1000.094.290852
ACACGAG800.090.62367
AACACGA800.090.62366
ACACGTG259.3776855E-486.998667
Produced by FastQC (version 0.11.5)