FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_225_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_225_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585043
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT55360.34926497262219386No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT47340.29866697622714333No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC33590.21191854101119023No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA33400.21071983536093344No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30540.19267616083601516No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT27450.1731814215765755No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC23880.1506583733059608No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23740.1497751165110347No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA21990.13873440657445885No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC21740.13715716229780517No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT21670.13671553390034213No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA21560.13602154641861453No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC21260.13412885328663007No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA20770.13103745450438886No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAACCAAG20050.1264949909876262No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA19680.12416066945817875No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA19030.12005983433887914No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18920.11936584685715151No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18510.11677916624343945No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA17710.11173198455814762No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT17680.11154271524494919No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC17430.10996547096829551No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA17410.10983929142616321No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC17310.10920839371550171No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA16380.10334104500635No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT16380.10334104500635No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC16010.10100672347690251No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15940.10056509507943949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCGA100.0070828145.057851
GCGAAGC100.0070854733145.039543
TTCCCGT100.0070854733145.039542
ACCGTCC100.0070854733145.039543
AGCACGC6400.0131.458681
CACGCAC6550.0128.431963
GCACGCA6550.0128.431962
GTAACAA12750.0121.150674
GAAACAC5350.0113.8628164
AAACACG5750.0107.196375
ACGCACT8150.0103.218324
CGCACTT8150.0103.211815
AAGCACG650.0100.424661
TAGAAAC7200.094.678592
AACACGT2600.092.03276
CAACGCA1600.090.623979
TCTACTA259.379957E-487.018235
CGGTGCA353.433253E-582.879732
ACACGTG2950.081.100787
ACGTGCA3600.080.577522
Produced by FastQC (version 0.11.5)