FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2018_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2018_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860301
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT112670.6056546763131342No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA98220.5279790743540964No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC94540.5081973293569159No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG89180.4793847877305877No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT82970.44600309304784547No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT77830.4183731557420009No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA64190.3450516878720164No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC61730.3318280213793359No Hit
GTAGTAACAAGAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA61310.329570322222049No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT60870.3272051135810818No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT58430.31408895657208163No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA56390.303122989236688No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA53630.2882866804888026No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT51420.27640688254212625No Hit
TAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCA50120.2694187661029049No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG47670.25624885435206457No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC46420.24952951162204398No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT46160.2481318883341997No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT45230.2431326973430644No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA43750.23517699555072002No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT42180.22673750108181417No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT39360.21157866388288776No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA38820.20867590782351889No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT38450.20668698237543282No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG38400.20641820866623195No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT37120.19953760171069088No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC36320.19523722236347774No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG36180.19448465597771544No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT34150.18357244338416204No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT33550.18034715887375213No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG33070.17776693126542426No Hit
GTGAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA32960.17717562910518242No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG32880.17674559117046112No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT32610.17529421314077667No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC32440.1743803825294939No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT32230.17325153295085044No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT30700.16502705744930524No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA30540.1641669815798626No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC29240.15717886514064122No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT29140.15664131772223958No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG28220.15169588147294444No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG27980.15040576766878047No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA27980.15040576766878047No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG27960.15029825818510015No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA27880.14986822025037883No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT27350.1470192189328501No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC26890.14454650080820255No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26710.1435789154550796No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA26190.140783668879391No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA25910.1392785361078664No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT24840.13352677873096883No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG24480.1315916080247229No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA24320.13073153215528024No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA24310.13067777741344008No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA24180.12997896576951792No Hit
CCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATG23970.1288501161908745No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23910.1285275877398335No Hit
GGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC23800.1279362855795917No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23360.12557107693862446No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22910.12315211355581704No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT22720.12213077346085392No Hit
GGGAAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA22470.12078690491484981No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA22420.12051813120564898No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT22130.11895924369228421No Hit
CTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC22090.11874422472492356No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT22000.11826043204836206No Hit
AGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAA21780.11707782772787845No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT21720.11675529927683745No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA21650.1163790160839563No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG21200.11396005270114891No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC21030.11304622208986609No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20520.1103047302560177No Hit
CTGTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTAC20300.10912212593553408No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20250.10885335222633327No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA20190.10853082377529227No Hit
CCTTTTCCCATCCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT20080.10793952161505048No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA19970.10734821945480866No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATA19780.10632687935984553No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA19700.10589684142512422No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT19440.10449921813727994No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19060.10245653794735368No Hit
GGTCTCAGCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAG18750.10079014095030858No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC18690.10046761249926758No Hit
TCTTTAGCCTGAATTTCTTTTTGGTAACTGTGTCTCAAAATTGATACCTC18670.10036010301558726No Hit
GGCCATGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATGTACCTGAA18630.10014508404822661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTATA100.0070802565145.075941
GCGAAGC100.007084242145.048633
AGCGAAG100.007084242145.048632
CCTCACC100.0070859506145.036945
AGCACGC4850.0124.1371461
GCACGCA5000.0120.3903662
CACGCAC5450.0110.449883
GAGCACG203.85854E-4108.806961
CGTGCTC203.866802E-4108.74847145
GAGCAGA1250.087.045561
ACGTGCA1550.084.221792
AACACGT1350.075.198276
AAGCGGA702.8030627E-972.537971
AAGCACG702.8030627E-972.537971
CGGTGCA502.3024295E-672.524322
CGGAGCG300.001933430872.524322
ACAACGC9400.070.185188
TAGTAAC23800.067.344022
CAACGCA9900.066.640479
GAAACAC4500.064.466064
Produced by FastQC (version 0.11.5)