FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_2017_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2017_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828217
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA118570.6485553957763219No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT102040.5581394331198102No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC84400.4616519811379065No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG78280.4281767426952052No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT75640.413736443759138No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT65180.3565222290351747No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA61790.33797957244681565No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG55470.30341037196350323No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC51810.28339086662031915No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA51150.27978079188630234No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT51080.27939790517208846No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA46390.2537444953197569No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG43170.23613170646591733No Hit
ACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAG42050.23000551903849487No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG41970.22956793422225044No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT41250.22562967087605026No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA40430.22114442650954455No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT39730.21731555936740551No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT39540.2162762954288249No Hit
GAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCT39310.2150182390821221No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT39300.21496354098009154No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT37180.2033675433496133No Hit
GTCTCTAGAATGTGAGGGAGCGGTCCCACTTTATTTTCAGGATCAGTGAG36980.20227358130900214No Hit
GTAGAAACACGAGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG35590.19467054512675466No Hit
GCTATGAACACAGCAAAAACAATGGATGGGATGGGAAAAGGAGAAGACGT35160.19231852673944067No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT34800.19034939506634058No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT34090.186465829822171No Hit
CTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAATGTGAGGG33910.18548126398562095No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT32770.17924568035413738No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG32590.17826111451758736No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA32090.17552620941605948No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC30780.16836075805005643No Hit
GTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGG30660.16770438082568973No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30450.16655572068304803No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC29730.16261745733684788No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT29520.16146879719420615No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG29490.1613047028881145No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29190.15966375982719774No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC29080.1590620807048616No Hit
GTAGAAACACGTGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG27990.15309998758353086No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27780.15195132744088913No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27330.14948991284951405No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA27280.14921642233936125No Hit
GGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGA27070.14806776219671952No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC26910.14719259256423062No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT26750.1463174229317417No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA26550.14522346089113056No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG26130.14292614060584713No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG25830.14128519754493038No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC25790.14106640513680815No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT25590.139972443096197No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA25490.13942546207589143No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT25480.1393707639738609No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA25290.13833150003528027No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT24810.1357059911378135No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA24720.1352137082195385No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC23950.13100195436318557No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA23730.12979859611851327No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA23420.128102954955566No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG23100.12635261569058814No Hit
AAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAG23060.12613382328246592No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT22800.12471167262967144No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT22550.12334422007890748No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22430.12268784285454079No Hit
AGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTG22200.12142978650783796No Hit
GAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAG22170.1212656922017463No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA22100.12088280548753239No Hit
GTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTC21780.11913246622255454No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT21560.11792910797788227No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT21080.1153035990804155No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA20950.11459252375401825No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA20850.11404554273371269No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG20550.11240459967279595No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT20490.1120764110606126No Hit
GTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGT20310.11109184522406255No Hit
CAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAAT20280.11092775091797089No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA19460.10644250655146517No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT19300.10556733691897625No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG19280.10545794071491514No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT19250.10529384640882347No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT19180.10491095969460955No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19070.10430928057227343No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGAAGTA18860.10316062042963171No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA18700.10228545079714278No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG18590.10168377167480665No Hit
GTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGAT18570.10157437547074553No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC18520.10130088496059277No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA18490.10113679065450107No Hit
CCATTTATGTTACAGGATGAGGACTCCAACCAGTGTCTTGCCCCAGAAGA18470.10102739445043997No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT18460.1009726963484094No Hit
AGTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCT18440.1008633001443483No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG18420.10075390394028719No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT18390.1005898096341955No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG18330.10026162102201215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAG100.007085234145.041783
GCCGAGC100.007085234145.041784
TAACACG151.228894E-4145.03785
ACCGTCC100.0070916107144.99814145
AGCACGC2950.0115.557561
GCACGCA2950.0115.5417562
CACGCAC3050.0111.7534943
GCGGTGC1300.094.8479841
CGGTGCG554.1472958E-1092.299322
TAGTAAC11550.090.415662
GGTGCAT502.2651875E-887.025063
CGAGACC259.3884795E-486.99888145
GGTGCGT608.276402E-1084.6077043
CGCACAT353.433597E-582.8787465
CCGGAGA801.8189894E-1281.561455145
GAAACAC16400.080.03834
AACAACG7950.079.33867
GTAGTAA13700.077.825031
TATCCTA755.638867E-1177.351386
ACAACGC8200.076.919758
Produced by FastQC (version 0.11.5)