FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2017_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2017_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839939
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA119190.6477932148837543No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT102670.5580076296007639No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC84740.4605587467845402No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG78730.42789462041948123No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT76090.4135463186551293No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT65480.3558813634582451No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA62090.33745683960174766No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG55700.30272742737666847No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC52030.28278111393910343No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA51520.2800092829164445No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT51360.27913968887012014No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA46630.2534323148756562No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG43410.23593173469337844No Hit
ACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAG42350.23017067413647951No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG42210.22940977934594572No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT41440.2252248579980097No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA40600.2206594892548068No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT39950.21712676344161408No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT39720.21587672200002284No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT39600.21522452646527954No Hit
GAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCT39510.2147353798142221No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT37400.20326760832831955No Hit
GTCTCTAGAATGTGAGGGAGCGGTCCCACTTTATTTTCAGGATCAGTGAG37190.20212626614251888No Hit
GTAGAAACACGAGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG35770.19440861898139014No Hit
GCTATGAACACAGCAAAAACAATGGATGGGATGGGAAAAGGAGAAGACGT35300.19185418647031233No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT34990.1901693480055589No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT34230.18603877628551815No Hit
CTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAATGTGAGGG34130.18549528000656543No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT33040.17957117056598074No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG32820.17837547875228474No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA32280.17544059884594No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC31000.16848384647534512No Hit
GTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGG30760.16717945540585855No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA30640.1665272598711153No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC29920.16261408666265567No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG29670.16125534596527386No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT29650.1611466467094833No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29340.15946180824472986No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC29320.1593531089889393No Hit
GTAGAAACACGTGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG28230.1534289995483546No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27930.15179851071149641No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27450.14918972857252333No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA27380.14880928117725642No Hit
GGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGA27190.14777663824724624No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC27060.1470700930846077No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT26980.1466352960614455No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA26750.14538525461985424No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG26260.14272212285298588No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG25960.1410916340161277No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC25890.1407111866208608No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT25710.1397328933187459No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA25600.1391350474118979No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT25580.13902634815610734No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA25420.138156754109783No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT25000.13587406973818156No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA24830.1349501260639619No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC24080.13087390397181645No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA23850.1296238625302252No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA23560.12804772332126227No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG23210.12614548634492773No Hit
AAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAG23190.12603678708913718No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT22970.1248410952754412No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT22660.12315625681068773No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22620.12293885829910665No Hit
AGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTG22360.12152576797382955No Hit
GAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAG22340.12141706871803902No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA22230.12081922281119103No Hit
GTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTC21920.11913438434643757No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT21720.11804739178853212No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT21280.11565600816114013No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA21060.11446031634744414No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA20920.1136994215569103No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG20690.11244938011531905No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT20590.11190588383636632No Hit
GTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGT20450.11114498904583249No Hit
CAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAAT20380.1107645416505656No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA19540.1061991729073627No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT19410.10549262774472415No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT19400.10543827811682886No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG19350.1051665299773525No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT19350.1051665299773525No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19180.10424258630313288No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGAAGTA19000.10326429300101796No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA18780.10206860118732197No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG18690.10157945453626452No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC18650.10136205602468341No Hit
GTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGAT18640.10130770639678816No Hit
AGTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCT18620.10119900714099762No Hit
CCATTTATGTTACAGGATGAGGACTCCAACCAGTGTCTTGCCCCAGAAGA18590.10103595825731179No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT18580.10098160862941652No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA18560.10087290937362597No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG18490.10049246197835907No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT18490.10049246197835907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG100.0070831287145.056215
AGCACGC3350.0125.608631
GCACGCA3450.0121.941262
CACGCAC3500.0120.199253
TATCCTA650.0100.415356
ATCCTAC650.0100.38267
GAACACG301.5960946E-596.7120364
GGTGCAT406.102455E-790.6675343
CCGACTT259.370527E-487.040834
CGAGACC608.2945917E-1084.581635145
TAGTAAC12800.084.434142
GAAGCGG1050.082.914061
TCCTACT801.8189894E-1281.560878
GAAACAC17100.081.441724
AAGCGGT651.5588739E-978.113572
ACACGTG7950.077.514177
AACACGT8050.076.576236
GTAGTAA14600.076.028221
TCCGACT300.00193240172.534033
AGTATAC300.00193240172.534033
Produced by FastQC (version 0.11.5)