FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2016_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2016_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901151
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA84380.44383639174373835No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG60930.3204900610209289No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT57990.30502574493030804No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC54440.2863528462494562No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA53570.2817766710797827No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC49580.26078938495679727No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA49050.25800160008331796No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG47390.24927004746072245No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG45030.2368565148165506No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT41320.21734202070219566No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG41240.21692122298544408No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40720.21418603782655873No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC40310.21202944952820685No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT40070.21076705637795207No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT38410.20203550375535664No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC37150.1954079397165191No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG32560.1712646707178967No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT31620.1663202975460655No Hit
GAATAAACACCCACATTCCAAACGTATTGAGACAATCACAGTTATAGTGT30600.16095512665748277No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC29540.1553795569105242No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA28830.15164497717435385No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA28710.15101378059922646No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28540.15011958545112933No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA27130.14270302569338258No Hit
ATATTGAGGGACGTGATGCAGATGTCAAAGGAAATCTACTCAAGATGATG26560.13970484196152752No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT26440.13907364538640013No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG26050.13702225651723612No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG25720.13528646593563584No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT25470.13397147307078713No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT24540.1290796996135499No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG23970.12608151588169483No Hit
TTCTTATAGGGCTGAAGTAGATGGTTTTATCATCTCTTACCATCTTGTAA23350.12282033357687001No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA23260.12234693614552447No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT23040.12118974242445761No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22650.1191383535552936No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22160.11656096754019013No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA21910.11524597467534141No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21860.11498297610237167No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG21830.11482517695858982No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC21700.1141413806688685No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT21300.11203739208511054No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC21040.11066979950566788No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20920.11003860293054049No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA20870.10977560435757076No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT20670.10872361006569178No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA20660.10867101035109783No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG20600.10835541206353413No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC20510.1078820146321886No Hit
TTCCAAACGTATTGAGACAATCACAGTTATAGTGTCTATTTTGTTGCTTT20200.1062514234797762No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA20070.10556762719005487No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT19930.10483123118573959No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT19580.1029902411749514No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA19570.10293764146035744No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG19290.10146484945172686No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19190.10093885230578739No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT19040.10014985658687815No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT100.007087093145.02924
ATTACGT100.0070915525144.99869145
CCCCGCC100.0070915525144.99869145
AGCACGC7550.0119.118761
CGGTGCA550.0118.660252
GCACGCA7650.0117.542
CACGCAC8100.0111.905253
ACACGTG2050.095.486947
CGTGCTC554.1654857E-1092.27189145
AACACGT2150.091.057666
GGTGCAT750.087.017523
CGCACTT11000.085.6945655
ACGCACT11150.084.546174
GGAGCGT353.434644E-582.8738254
ACGAGCG353.4382305E-582.856399
AAACACG6500.081.435195
ACGTGCA1400.077.6778649
GCGGTGC2150.077.588051
GAAACAC7050.076.114624
CAACGCA3550.075.56279
Produced by FastQC (version 0.11.5)