FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2015_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2015_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256082
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA26061.0176427862950148No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG14580.5693488804367351No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA13680.5342038878171836No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC12720.49671589568966196No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG12690.4955443959356769No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA12380.4834388984778313No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC11680.4561039042181801No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA11500.44907490569426983No Hit
AGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTC10580.4131489132387282No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT9190.35886942463742083No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG8430.3291914308697995No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT8010.31279043431400877No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG7500.2928749384962629No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA7350.2870174397263377No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA7170.27998844120242733No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC6820.26632094407260176No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA6750.26358744464663664No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT6520.25460594653275126No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT6330.24718644809084592No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6290.24562444841886583No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG6260.24445294866488076No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG6220.2428909489929007No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT6200.2421099491569107No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG6150.24015744956693558No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA5920.2311759514530502No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT5850.22844245202708507No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC5760.22492795276512992No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT5760.22492795276512992No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT5620.21946095391319967No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT5470.21360345514327442No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATTA5260.20540295686537904No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT5220.20384095719339898No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC5210.20345045727540398No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA5160.20149795768542889No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT5100.19915495817745876No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT5100.19915495817745876No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT5060.1975929585054787No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT4950.1932974594075335No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA4730.18470646121164314No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT4590.17923946235971291No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA4560.17806796260572785No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT4450.17377246350778267No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAGCTA4450.17377246350778267No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG4430.17299146367179263No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA4410.17221046383580257No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC4390.17142946399981257No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT4300.16791496473785741No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG4230.1651814653118923No Hit
CTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTA4140.16166696604993713No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG4060.158542966705977No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA3930.15346646777204176No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT3890.1519044681000617No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC3840.14995196851008663No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT3830.1495614685920916No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA3680.14370396982216635No Hit
CTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGG3610.1409704703962012No Hit
CATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATAC3580.13979897064221616No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC3550.13862747088823113No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT3540.1382369709702361No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT3530.13784647105224107No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT3450.13472247170828094No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC3370.1315984723643208No Hit
CAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAGCAGCAGTT3320.1296459727743457No Hit
AAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG3320.1296459727743457No Hit
TCTTTAAATGATGATGGATTGGATAACCATACTATACTGCTCTATTACTC3320.1296459727743457No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA3240.12652197343038557No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC3210.1253504736764005No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA3110.12144547449645035No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG3100.12105497457845535No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAG3080.12027397474246529No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC2900.11324497621855499No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT2820.11012097687459485No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT2790.1089494771206098No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT2780.1085589772026148No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG2770.10816847728461977No Hit
ACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA2750.10738747744862974No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT2720.10621597769464469No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT2710.10582547777664966No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA2650.10348247826867957No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2650.10348247826867957No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT2640.10309197835068455No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA2630.10270147843268952No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA2580.10074897884271444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA100.0070838663145.026952
GGTGCAT100.0070838663145.026953
GCGTTTT100.007088004144.998636
AGCACGC351.88993E-9124.357391
CACGCAC351.893568E-9124.3088153
GCACGCA351.893568E-9124.3088152
GCGGTGC203.8538856E-4108.812711
GAAACAC650.0100.403274
CTTTAAG301.5972515E-596.665769
AAACACG700.093.231615
TAGTAAC5300.079.354372
TAGTAGA651.5552359E-978.091432
AACACGA755.4569682E-1177.33266
TAGAAAC3300.076.9082263
GTAACAA5550.074.4733054
ACACGAG809.822543E-1172.499317
GTACTAT300.001934164872.499316
AAACAAC3650.069.519896
GTAGTAA6700.066.045531
ACAACGC3700.064.661558
Produced by FastQC (version 0.11.5)