FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2015_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2015_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824906
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA186051.019504566262591No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG106920.5858931912109445No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA100230.5492337687530208No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC88730.48621682431862245No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG88710.4861072296326496No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA85570.4689008639349095No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA85550.46879126924893666No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC85030.4659418074136421No Hit
AGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTC76520.41930926853218736No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT63800.3496070482534443No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG60610.33212669584077203No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT58560.3208932405285532No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA55120.3020429545412202No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG52350.2868640905339782No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA49450.2709728610679126No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC47180.2585338642099922No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT46250.2534377113122539No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45120.24724561155478694No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT45060.2469168274968683No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA44630.2445605417484517No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG44550.24412216300456024No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG43810.24006715962356417No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG43590.23886161807786266No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT43580.2388068207348762No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA41700.22850492025342675No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT40740.22324437532672917No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC39890.21858660117288234No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT39700.21754545165614012No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT39220.21491517919279132No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT38040.20844909272039217No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATTA37730.20675037508781274No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT37050.20302415576473526No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC36660.2008870593882644No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT36580.20044868064437293No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA35660.19540732508962105No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT35430.19414698620093307No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT35070.1921742818534215No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT34900.19124272702265213No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA32640.1788585275077182No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA31520.17272122509323767No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT30910.16937858717106524No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA30880.16921419514210595No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG30320.1661455439348657No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT30030.16455642098825912No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG29940.16406324490138122No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAGCTA29480.16154256712400528No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT28660.15704918499911777No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC28530.15633681954029413No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC28520.1562820221973077No Hit
CTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTA28470.15600803548237555No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG28450.15589844079640267No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT28270.15491208862264688No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA26870.1472404606045462No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26840.1470760685755869No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT26690.14625410843079042No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT25580.14017160335929632No Hit
CTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGG25540.1399524139873506No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC25100.13754133089594753No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC24870.13628099200725954No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23740.1300888922497926No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC23610.12937652679096895No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT23450.12849976930318605No Hit
CATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATAC23410.12828057993124029No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA23290.1276230118154031No Hit
AAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG23020.1261434835547694No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA22760.12471875263712212No Hit
CAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAGCAGCAGTT22450.1230200350045427No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG22370.12258165626065123No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAG21760.1192390183384788No Hit
TCTTTAAATGATGATGGATTGGATAACCATACTATACTGCTCTATTACTC21060.11540320432942848No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT20550.11260853983712038No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC20400.11178657969232388No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT20160.11047144346064948No Hit
ACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA19850.10877272582807006No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT19290.10570407462082979No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG19230.10537529056291119No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA18970.10395055964526392No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18970.10395055964526392No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC18740.10269022075657597No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT18510.101429881867888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAGT100.007077762145.092971
AGTGTAT100.0070858872145.03736
AGCACGC5850.0131.451751
CGGTGCA450.0128.936172
CACGCAC6100.0128.407753
GCACGCA6050.0128.270192
GGTGCAT501.8189894E-12116.0425643
GAAACAC3000.094.2845844
CGGTGCC406.1061473E-790.658252
TGACTAA259.388625E-486.998535145
AAACACG3450.084.086515
GCGGTGC1950.081.847321
TAGAAAC22350.081.125953
GAGCAGA1200.078.5920261
TAGTAAC39550.075.18562
AAGCGGA1000.072.52662
ACGTGCA1700.072.52662
AAACAAC24750.072.3721546
GTAACAA40950.071.198274
AACAACG25000.070.468817
Produced by FastQC (version 0.11.5)