FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_2012_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_2012_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491089
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC94790.6357098737902298No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT89360.5992935364689834No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG80060.5369230139850807No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT66140.44356842549304565No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA65190.43719724308877606No Hit
GGTTTATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA65070.43639246215349986No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG62370.4182848911097862No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT59830.40125036131310743No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC55880.3747596555269337No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG51870.3478665592731218No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC49380.33116735486614146No Hit
GCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA48410.3246620423059925No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT47500.31855912021348154No Hit
CCTTATTTCCTCAAATTTCCGTCCCAATTGTTCCCGCCACTTCTCATTTC42450.284691255853943No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA40210.2696686783954546No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT39410.26430347216028016No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT39350.2639010816926421No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT36920.24760426775329974No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT35240.23633733465943346No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT34360.2304356078007416No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT34220.22949669670958606No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC33490.2246009460199894No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA32140.21554716049813258No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT31760.21299868753642473No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCA30520.20468261787190437No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA30400.2038778369366282No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATT29130.1953605720382888No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT29030.194689921258892No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC28910.19388514032361584No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT28450.19080014673839052No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA28340.19006243088105407No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG28020.18791634838698426No Hit
GTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT27790.18637385159437164No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG27490.18436189925618124No Hit
GGCATAAGCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA27380.18362418339884473No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA27060.18147810090477495No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT27010.18114277551507657No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG26910.18047212473567975No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC25760.17275964077261652No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG25420.1704794281226674No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC25410.1704123630447277No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25350.17000997257708964No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA25310.1697417122653309No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT25250.16933932179769282No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA25240.16927225671975313No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC25060.1680650853168389No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG25010.1677297599271405No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCCAGT24940.16726030438156272No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC24860.16672378375804528No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA24760.1660531329786485No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC23810.15968195057437887No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA23750.15927956010674077No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATG23520.15773706331412815No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA23410.15699934745679164No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA23180.155456850664179No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT22810.15297544278041084No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG22720.1523718570789537No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC22640.15183533645543626No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC22610.15163414122161722No Hit
GTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACCTTGAT22600.15156707614367754No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG22110.1482808873246332No Hit
GTTGAAGATTATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA21680.14539708897322695No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC21550.1445252429600111No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT21540.1444581778820714No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC21490.14412285249237303No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC21370.14331807155709686No Hit
ATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGACCGCCTGGTC21340.14311687632327783No Hit
ACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACC21260.14258035569976038No Hit
GAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGA21250.1425132906218207No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC21040.14110492398508742No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG20810.13956242719247475No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG20740.139092971646897No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC20710.13889177641307796No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG20650.13848938594543989No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC20520.13761753993222403No Hit
CCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATGCCTAGGCAAAAG20300.13614210821755104No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC20070.13459961142493843No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG19830.1329900495543861No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT19830.1329900495543861No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT19740.13238646385292896No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA19550.13111222737207504No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT19520.130911032138256No Hit
ATACAGAGATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACT19470.1305757067485576No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG19390.13003918612504017No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA19390.13003918612504017No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAA19070.12789310363097037No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCAATTGT19060.1278260385530307No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG19050.12775897347509102No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT18940.12702125761775454No Hit
GTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAGCTGTGATGA18910.1268200623839355No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC18760.12581408621484028No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAAATCCATTCCACAATTTGT18590.12467397988986573No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC18590.12467397988986573No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT18400.12339974340901179No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA18390.12333267833107213No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA18360.12313148309725308No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG17970.12051594505760556No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA17820.11950996888851036No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT17770.11917464349881193No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC17710.11877225303117385No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTT17710.11877225303117385No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG17440.11696149592680248No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG17400.11669323561504377No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA17390.11662617053710408No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT17330.11622378006946602No Hit
CTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACA17250.11568725944594857No Hit
TTCCAGTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAA17220.11548606421212954No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA17190.11528486897831049No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC17110.11474834835479303No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA17090.11461421819891368No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT17070.11448008804303433No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC16920.11347411187393912No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG16900.11333998171805974No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC16900.11333998171805974No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC16770.11246813570484392No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT16650.11166335476956776No Hit
CTTCAGTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTT16630.11152922461368839No Hit
TCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGGT16620.11146215953574871No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT16580.11119389922398999No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT16450.11032205321077414No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT16370.1097855325872567No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCC16360.10971846750931702No Hit
TAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTA15950.10696879931379012No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAGAAGTGGCGGGAA15930.10683466915791075No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG15840.10623108345645363No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC15350.10294489463740931No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG15340.10287782955946963No Hit
CTATTATCTTTTGCCTAGGCATAAGCATAAACCAGTCTCGTGACATTTCC15210.10200598354625377No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC15200.1019389184683141No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG15160.10167065815655538No Hit
TGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTT14940.1001952264418824No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT14920.10006109628600306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGC100.0070914836144.99812145
CCTCGCC151.2301892E-4144.99812145
GAGCGAA600.0108.817921
CGAAAGC5200.097.621073
GCGAAAG5150.097.160732
AGTAGAA5900.084.8410951
TTCTACT1600.081.583334
GTTTAAA451.2286146E-680.605871
GTAGAAA13200.080.239481
GTACTGA755.638867E-1177.347896
TTTCTAC1800.072.518523
TGGCTTC502.3069733E-672.49906145
TACTAAT2150.070.8130347
TAGAAAC15500.068.307762
AGAAACA15950.066.3805853
ATATAGT1450.065.0405961
GGTACTT350.003567030262.142057
CAGGTAC954.5474735E-1061.0682263
ATTGGGG1200.060.415882145
ATAGTGG1600.058.921293
Produced by FastQC (version 0.11.5)