FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_164_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_164_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715769
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT105720.6161668616229807No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT64910.37831433019246763No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT44000.2564447778226556No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA34490.20101773607053164No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33010.19239186627104232No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT29120.16971981659535754No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC28880.16832102689814304No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA28010.16325041424574055No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC27780.16190990745257666No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC24950.14541584560625584No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT23220.13533290320550143No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA23160.13498320578119782No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA22940.13370098189208454No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC22150.12909663247208686No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA21980.1281058231032266No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG21890.12758127696677116No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT21760.12682359921411332No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT21300.12414258562778557No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC20480.11936338749563608No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG20210.11778974908626977No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT19990.11650752519715649No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT19700.11481732097968898No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC19510.11370994580272753No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT19170.111728327065007No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAACCAAG18680.10887246476652744No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC18570.10823135282197079No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18450.10753195797336354No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA18220.10619145118019965No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18160.10584175375589605No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17500.10199508208855622No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC17340.10106255562374657No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA17260.10059629239134174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGC100.007083008145.056764
CCCCGTG100.007083008145.056762
CTAAACG100.007083008145.056762
CACAGCG100.007083008145.056763
CGTGGCC100.0070842425145.048315
CGGTGCA1450.0135.052842
AGCACGC12050.0133.034361
GCACGCA12250.0130.847122
CACGCAC12300.0130.315223
GAGCAGA1300.0128.334411
GTAACAA14850.0124.5436864
GAAACAC5900.0111.865814
GAGCCCC203.8667902E-4108.748184145
ACGCACT14950.0108.671284
AAACACG6100.0108.191775
CGCACTT15150.0107.230435
TAGAAAC8600.086.865392
GCACTTT23150.080.82376
ACGTGCA3200.079.327922
GGTGCGT1300.078.107493
Produced by FastQC (version 0.11.5)