FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_1208_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_1208_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513571
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT60310.39846165128692346No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT59260.39152441477803157No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT51370.339396037582644No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC51240.338537141633924No Hit
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG43680.28858903876990244No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA41910.2768948400834847No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA41000.27088256844244507No Hit
ATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTC40920.2703540170893866No Hit
GTTTTATCGTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTT40530.2677773292432268No Hit
GTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGCATCAAGGAAA37790.24967444540097555No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT37730.24927803188618175No Hit
ACAACAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTC36510.24121762375204073No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA35780.23639459265538254No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA34780.22978770074215216No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA32860.21710246826874988No Hit
AGTAGAAACAACGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG32860.21710246826874988No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT31920.2108919898703133No Hit
ACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCGTCAGGGTGGA29400.1942426222489728No Hit
CTCCTGTGCTAAATGAAAAAAAAAAGAAAAAGAAAATATGACCCTACCTA29270.19338372630025286No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG29070.19206234791760676No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC28040.1852572492469795No Hit
ATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAAA27800.1836715951878042No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT27170.17950925328246908No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT26740.17666828975978002No Hit
GGCATATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCT26590.17567725597279546No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG26360.17415767083275246No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA26360.17415767083275246No Hit
GGATATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTT26230.17329877488403253No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC26020.17191132758225414No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA25130.16603119377947914No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC23860.15764044104967656No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA23220.15341203022520913No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA23190.1532138234678122No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC22930.15149603157037234No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG22540.14891934372421248No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT22540.14891934372421248No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22210.14673906939284645No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT20640.13636624908907477No Hit
ATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCTCTGGG19850.1311468044776228No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT19740.13042004636716745No Hit
GTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGC19610.1295611504184475No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC19470.12863618555059525No Hit
AATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAA18960.12526667067484776No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT18820.1243417058069955No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC18800.1242095679687309No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG18730.12374708553480476No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA18600.12288818958608481No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG18460.12196322471823258No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA18290.1208400530929834No Hit
ATATGACCCTACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCG18030.11912226119554352No Hit
GTAGTAACAAGAGCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC17940.11852764092335277No Hit
CCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGT17760.11733840037897134No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC17400.11495991929020839No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT17360.11469564361367918No Hit
AAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACT17290.11423316117975305No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT17150.1133081963119008No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT17040.11258143820144545No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT16750.11066543954660864No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT16590.1096083368404918No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT16470.10881550981090414No Hit
ATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGTCCACC16400.10835302737697801No Hit
GAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACTGTTTTATCGTA16090.1063048908838766No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA16040.1059745462882151No Hit
CCCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAG15970.10551206385428896No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA15660.10346392736118755No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15410.10181220438287995No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA15400.10174613546374767No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA15310.10115151519155692No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG15310.10115151519155692No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA15220.10055689491936619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG100.007077606145.093231
CATTAGT100.007077606145.093231
CACGCAC4600.0124.562093
GTAACAA27300.094.581024
GAGACTA406.1196624E-790.623344145
TAGTAAC29550.088.361212
TAACGTG259.376051E-487.027166
AACGTGC259.388366E-486.998417
CGTGCGG259.388366E-486.998419
CAGCCCG801.8189894E-1281.590665
ACGTGCA4250.078.502882
CGGTGCA1300.078.109052
TAACAAG33100.078.0027545
TATATAA300.001932778672.529832
TATAACG300.001932778672.529834
CAGCTGG8750.072.110615
AGTAACA36300.071.9304053
CAACGCA12800.069.10039
CGGAGCA1300.066.950612
AACAACG13400.066.006267
Produced by FastQC (version 0.11.5)