FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_1208_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_1208_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524973
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT60680.3979086842849021No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT59640.3910888914098807No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT51660.33876009608038965No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC51490.33764532224504956No Hit
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG43960.2882673988326351No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA42270.2771852354107253No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA41310.2708900419876286No Hit
ATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTC41120.26964411828930746No Hit
GTTTTATCGTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTT40840.2678080202075709No Hit
GTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGCATCAAGGAAA38060.24957818925318678No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT37970.24898801486977146No Hit
ACAACAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTC36790.2412501729538818No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA36000.23606975336612515No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA34950.22918438555961318No Hit
AGTAGAAACAACGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG33090.21698744830236338No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA33040.21665957364491042No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT32170.21095455460522905No Hit
CTCCTGTGCTAAATGAAAAAAAAAAGAAAAAGAAAATATGACCCTACCTA29600.19410179721214735No Hit
ACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCGTCAGGGTGGA29590.19403622228065676No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG29250.1918066746099767No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC28150.18459343214601176No Hit
ATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAAA28050.18393768283110587No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT27340.17928186269527396No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT26930.17659329050415976No Hit
GGCATATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCT26750.17541294173732913No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG26550.17410144310751732No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA26470.1735768436555926No Hit
GGATATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTT26390.17305224420366788No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC26180.17167517064236548No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA25360.16629802626013707No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC24020.15751098544039796No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA23380.15331418982500017No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA23350.15311746503052842No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC23160.1518715413322072No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG22720.14898624434662122No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT22660.14859279475767767No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22300.14623209722401642No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT20850.13672373215788083No Hit
ATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCTCTGGG20000.13114986298118067No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT19840.1301006640773312No Hit
GTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGC19750.12951048969391588No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC19550.1281989910641041No Hit
AATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAA19160.12564156873597107No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT19010.1246579447636122No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC18920.1240677703801969No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG18790.12321529627081923No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA18740.12288742161336627No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG18550.12164149791504504No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA18460.12105132353162973No Hit
ATATGACCCTACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCG18100.11869062599796848No Hit
GTAGTAACAAGAGCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC17970.11783815188859083No Hit
CCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGT17910.11744470229964726No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC17550.11508400476598601No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT17460.1144938303825707No Hit
AAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACT17390.11403480586213657No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT17310.11351020641021187No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT17180.11265773230083417No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT16900.11082163421909765No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT16670.10931341079481408No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT16560.10859208654841758No Hit
ATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGTCCACC16520.10832978682245521No Hit
GAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACTGTTTTATCGTA16190.10616581408326574No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA16130.10577236449432219No Hit
CCCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAG16120.1057067895628316No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA15770.10341166696066094No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA15560.10203459339935854No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15510.1017067187419056No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG15450.10131326915296206No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA15400.1009853944955091No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA15370.10078866970103734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC5700.0115.792923
GTAACAA29700.096.7061844
TAGTAAC31200.092.289312
GGAGCGT259.3724695E-487.035563
TAACAAG35250.081.6778265
GCACGCA8850.074.57852
AGTAACA39400.073.818243
CAGCTGG7000.071.4864655
CAACGCA13200.069.203329
ACGTGCT6550.068.654012
CGGTGCA1400.067.3489462
AACAACG13950.065.482727
AGCACGC10300.064.798641
GTATAGG902.8012437E-1064.4855961
TATAGGT902.8194336E-1064.4707952
GTAGTAA46050.064.1180041
GTGCTAG6200.062.001144
AACACGT9100.060.5662546
GTGCTCC851.3016688E-859.730294
TGGACGA8150.058.71062145
Produced by FastQC (version 0.11.5)